Device and method for magnetic separation of biological molecule

Details Device and method for magnetic separation of biological molecule Device and method for magnetic separation of biological molecule

1998-05-15

2000-08-29

Reifsnyder, David A.

Liquid purification or separation

Processes

Using magnetic force

210222, 210223, 209213, 96 1, 435 2, 435 721, 4351731, 435261, 435803, 436526, 436824, B01D 3506, B03C 100, C12N 1300

Patent

active

061103802

ABSTRACT:
Provided is a magnetic separation device comprising a container having one or more outer surfaces; at least one flexible magnetic sheet; and a non-permanent adhesive that is used to detachably secure an outer surface of the container to a flexible magnetic sheet. A method of using the magnetic separation device according to the present invention comprises obtaining a fluid containing a mixed population of biological molecules, from which it is desired to separate at least one subpopulation of biological molecules; mixing the fluid with a magnetic separation reagent; contacting the mixture with the fluid holding chamber of the magnetic separation device; incubating the mixture for a sufficient time to allow for complexes to form between the subpopulation of biological molecules and the magnetic separation reagent; positioning the magnetic separation device in a position that magnetically attracts the complexes towards the flexible magnetic sheet, and thereby holds them in position in the container; and removing the remainder of the fluid from the magnetic separation device.

REFERENCES:
patent: 4910148 (1990-03-01), Sorensen et al.
patent: 5078871 (1992-01-01), McCready
patent: 5663241 (1997-09-01), Takamatsu et al.

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