Device and method for DNA amplification and assay

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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422 50, 435 6, 435 911, 435183, 4352883, 4353051, C12M 100, C12M 140, G01N 3300, C12Q 168

Patent

active

059486732

ABSTRACT:
A DNA amplification and homogeneous DNA probe assay device is provided which includes a multiplicity of discrete sample cells in a flat "card" format, with each sample cell containing the reagents necessary for both DNA amplification and homogeneous DNA probe assay. The device is particularly suitable for fluorescence polarization DNA probe assays, aid is preferably provided with and integal polarizer to avoid the need for polarizing elements in the related measuring apparatus. The size and geometry of the sample cells allows for a "hot start" of the DNA amplification reaction and thereby avoids mis-priming of the amplification reaction.

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