Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1989-03-08
1990-07-17
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041823, 204299R, 2041821, G01N 2726, G01N 2728
Patent
active
049419587
ABSTRACT:
A device and method for sensing analyte ionic components are described. The device includes at least one detecting element including a first end, a second end, a first ion exchange medium extending from the first end to the second end, and a second ion exchange medium, which is in fluid communication with and has higher ion mobility than the first medium. The second medium includes an ion concentration member covered by a selectively permeable membrane. An analyte introduced at the ion concentration member passes therethrough and disassociates into its ionic species which collect in the ion concentration member. After collecting for a sufficient length of time, a voltage is applied so that the analyte ions move from the second medium into the first medium and toward a second end of the element at a rate determined by the applied voltage and their individual mobilities. Ions with different mobilities separate spatially and pass through the second end of the element at different times. The separated ions can then be detected by a variety of known techniques. The device can be made by photolithography methods. The method of detecting includes the steps of: collecting the ionic components of the analyte in the ion concentration member; effecting an electric field in the electrolytic solution to cause the ionic components to migrate from the first end of the element to the second end; and detecting the migration from the first to the second end of the element.
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J. W. Jorgenson and K. D. Lukacs, "Zone Electrophoresis in Open-Tubular Glass Capillaries," Analytical Chemistry, 1981, 53, 1298-1302.
F. E. P. Mikkers, F. M. Everaerts, and Th. P. E. M. Verheggen, Journal of Chromatography, 1979, 169, 11-20.
Bach K.
Niebling John F.
Phasge Arun S,.
Westinghouse Electric Corp.
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