Refrigeration – Processes – Treating an article
Patent
1982-09-27
1984-06-26
Capossela, Ronald C.
Refrigeration
Processes
Treating an article
62 78, F25D 1702
Patent
active
044558422
ABSTRACT:
An enclosed device and method for controlled freezing of cell cultures, especially eucaryotic cell cultures, contained in cell culture vials by means of a conventional freezer includes a container and top, a supporting grid and supporting grid base within the container for supporting the culture vials and a compatible liquid refrigerant in a volume of at least fifteen times the culture volume and at a level to totally immerse all of the cultures to be frozen. Compatible liquid refrigerants include the straight chain aliphatic alcohols, preferrably ethanol and methanol.
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Biotech Research Laboratories, Inc.
Capossela Ronald C.
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