Device and method for controlled freezing of cell cultures

Refrigeration – Cooled enclosure – Portable receptacle

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62 78, 435284, F25D 308

Patent

active

043770770

ABSTRACT:
An enclosed device and method for controlled freezing of cell cultures, especially eucaryotic cell cultures, contained in cell culture vials by means of a conventional freezer includes a container and top, a supporting grid and supporting grid base within the container for supporting the cultire vials and a compatible liquid refrigerant in a volume of at least fifteen times the culture volume and at a level to totally immerse all of the cultures to be frozen. Compatible liquid refrigerants include the straight chain aliphatic alcohols, preferrably ethanol and methanol.

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patent: 4226940 (1980-10-01), Storrs
"Instructions for BF-5 Biological Freezer", Form 11-361-B, Mar. 1975, pp. 1-4.
"Planer Mini-Freezer R202/200R", Advertising Literature, four pp., undated.

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