Device and method for automated separation of a sample of whole

Chemistry: analytical and immunological testing – Automated chemical analysis – Utilizing a centrifuge or compartmented rotor

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422 72, 422101, 422102, 436 63, 436177, 494 16, B04B 502, B04B 1100, B01D 2126

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active

048472050

ABSTRACT:
A device and a method for automated processing and separation of an unmeasured sample of whole blood into multiple aliquots of plasma. Capillaries are radially oriented on a rotor, with the rotor defining a sample chamber, transfer channels, overflow chamber, overflow channel, vent channel, cell chambers, and processing chambers. A sample of whole blood is placed in the sample chamber, and when the rotor is rotated, the blood moves outward through the transfer channels to the processing chambers where the blood is centrifugally separated into a solid cellular component and a liquid plasma component. When the rotor speed is decreased, the plasma component backfills the capillaries resulting in uniform aliquots of plasma which may be used for subsequent analytical procedures.

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patent: 4740472 (1988-04-01), Bartis et al.
Burtis et al., "Development of a Simple Device for Processing Whole-Blood Samples . . . ", 32 Clinical Chem. 1672 (1986).

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