Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1995-08-18
1997-07-08
Marschel, Ardin H.
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
422 50, 422 52, 422 55, 422 57, 422 58, 422 61, 422 63, 422 69, 435 6, 435 911, 435 912, 4352831, 4352871, 4352872, 4352873, 4352881, 4352882, 4352887, 4352891, 4352901, 4352904, 4352931, 4353041, 435810, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88, C12Q 168, C12M 140, C12P 1934
Patent
active
056458010
ABSTRACT:
Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.
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patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5229297 (1993-07-01), Schnipelsky et al.
Higuchi, Russell, Simultaneous Amplification and Detection of Specific DNA Sequences, Bio/Technology, Research, vol. 10, Apr. 1992, pp. 413-417.
Higuchi, Russell, Kinetic PCR Analysis: Real-Time Monitoring of DNA Amplification Reactions, Bio/Technology, Research, vol. 11, Sep. 11, 1993, pp. 1026-1030.
Bouma Stanley R.
Coules Ronald A.
Gordon Julian
Shain Eric B.
Solomon Natalie A.
Abbott Laboratories
Brainard Thomas D.
Marschel Ardin H.
Yasger Paul D.
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