Developmental marker gene of CD4-CD8 thymocytes

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 231, 536 235, 435 711, 4351723, 4353201, 530350, 935 11, 935 22, C07H 2104, C12N 1512, C12N 1563

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053127331

ABSTRACT:
The present invention provides novel DNA sequences, recombinant DNA (rDNA) molecules, processes for producing novel T-cell proteins expressed in T-cell development, the novel T-cell proteins in substantially pure form and antibodies which bind to the novel proteins. More particularly, it relates to novel DNA sequences expressed in appropriate hosts and the novel T-cell proteins produced in these hosts. The present invention also provides novel transmembrane proteins in substantially pure form, rDNA molecules encoding transmembrane proteins and processes for producing the novel transmembrane proteins. The DNA sequences and recombinant DNA molecules of this invention are characterized in that they are expressed by T lymphoma cells and have at least one of the following characteristics: (1) is expressed in normal thymus, activated spleen cells, or gut associated lymphoid tissue, (2) is expressed in ovarian tissue, normal liver and/or in a stage specific manner in embryonic development and (3) encode novel transmembrane proteins having multiple membrane spanning domains.

REFERENCES:
MacCleod et al, Cell Growth & Differentation, 1: 271-279 (1990), "Isolation of . . . cDNA from T Lymphoma Cells".
Hedrick et al, Nature 308: 149-153 Isolation of cDNA Clones Encoding T Cell Specific. . . .
Hays et al., Int. J. Cancer 38: 597-601 (1986) Tumorgenidity of T Lymphoma/T Lymphoma Hybrids . . . .
McMichael et al, from Leukocyte Typing III, Oxford Univ. Press (1987) pp. 31-62, vol. 5.1.

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