Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-12-24
1998-07-07
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C12N 1500
Patent
active
057766860
ABSTRACT:
The polymerase chain reaction (PCR) was used to identify Tilletia indica, the causal agent of Karnal bunt of wheat. The method uses two sets of oligonucleotide primers developed by sequence analysis of cloned Dra I fragments of mitochondrial DNA of T. indica. The primer pair TI17M1 (5'-TCCCCTTGGATCAGAACGTA-3') and TI17M2 (5'-AGAAGTCTAACTCCCCCCTCT-3'), derived from clone pTI-MD17, amplified a single 825-bp product from all isolates of T. indica and no products for other Tilletia species. In addition, the primer pair TI57M1 (5'-TTTTCCCTCTCTC-CTTTTTTCA-3') and TI57M2 (5'-AGCAAAGACAAAGTAGGCTTCC-3'), derived from clone pTI-MD57, produced a product of 118 bp which was unique to T. indica.
REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
Armour et al., Human Molecular Genetics 3(43): 599-605 (Mar. 1994).
Genbank Accession No. X75409.
Ferreira et al., Phytopathology 85(10): Abstract No. 490, p. 1175 (1995).
Smith et al., Phytopathology 84(10): Abstract No. 679, p. 1152 (1994).
Bej et al. Critical Reviews in Biochemnistry and Molecular Biology 26(3/4) :301-334 (1991).
Steffan et al., Annual Reviews in Microbiology 45 : 137-161(1991).
Beck Raymond J.
Bonde Morris R.
Peterson Gary L.
Schaad Norman W.
Smith Oney P.
Deck Randall E.
Fado John D.
Jones W. Gary
Silverstein M. Howard
The United States of America as represented by the Secretary of
LandOfFree
Development of a PCR-based method for identification of Tilletia does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Development of a PCR-based method for identification of Tilletia, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Development of a PCR-based method for identification of Tilletia will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1204090