Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Reexamination Certificate
2004-06-24
2010-06-08
Para, Annette H (Department: 1661)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
C435S420000, C435S430100, C435S410000, C800S295000, C800S298000
Reexamination Certificate
active
07732205
ABSTRACT:
The invention provides methods for producing pine somatic embryos using a liquid development medium and/or a liquid stratification medium. In a first aspect, the methods comprise the step of culturing embryogenic cells in, or on, a liquid development medium to produce cotyledonary pine somatic embryos. In another aspect, the methods comprise the step of culturing pine cotyledonary somatic embryos in, or on, a liquid stratification medium to produce stratified cotyledonary somatic embryos. The invention also provides methods for producing pine somatic embryos in bioreactors.
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Gupta Pramod K.
Holmstrom Diane G.
Larson Bonnie
Zucati Judith
Christensen O'Connor Johnson & Kindness
Para Annette H
Weyerhaeuser NR Company
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