Detritylation of DMT-oligonucleotides using cationic ion-exchang

Details Detritylation of DMT-oligonucleotides using cationic ion-exchang Detritylation of DMT-oligonucleotides using cationic ion-exchang

1995-05-23

1998-09-15

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, C07H 100, C07H 2104, C07H 2100

Patent

active

058080422

ABSTRACT:
The present invention comprises a method for quickly and efficiently detritylating oligonucleotides synthesized by standard chemical techniques. The method comprises contacting a 5'-DMT oligonucleotide with the H.sup.+ form of a "DOWEX," "AMBERLYST" or "AMBERLITE" ion-exchange resin for about 10 minutes to about 2 hours. The method is particularly advantageous when used in large scale synthesis. The method is quicker than the standard acetic acid method and eliminates much of the post-detritylation processing associated with the acetic acid method.

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