Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-08-21
2007-08-21
Naff, David M. (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S177000, C436S528000, C530S402000, C530S413000, C530S812000, C536S022100
Reexamination Certificate
active
10746565
ABSTRACT:
A competitive binding assay is used to determine whether a non-nucleic acid molecule from a library of non-nucleic acid molecules binds to a target. The non-nucleic acid molecule competes with a labeled nucleic acid ligand for binding to the target which may be immobilized. Detecting displacement of labeled nucleic acid ligand from a complex of the labeled nucleic acid ligand and the target determines binding of the non-nucleic acid molecule to the target. The nucleic acid ligand may be immobilized and contacted with a labeled target to form a complex. Adding a non-nucleic acid molecule to the complex displaces labeled target from the complex, and detecting displacement of the labeled target determines binding of the non-nucleic acid molecule to the target. Labeled nucleic acid ligand or labeled target displaced from or remaining in the complex can be detected for detecting displacement. Nucleic acid ligands that bind to the target are identified by the SELEX method. The assay provides a high throughput screening method for determining whether a non-nucleic acid molecule binds to a target.
REFERENCES:
patent: 5270163 (1993-12-01), Tuerk et al.
patent: 5580737 (1996-12-01), Polisky
patent: 5712096 (1998-01-01), Purohit et al.
patent: 5723323 (1998-03-01), Kauffman
patent: 5756291 (1998-05-01), Griffin et al.
patent: 5780228 (1998-07-01), Parma
patent: 5783397 (1998-07-01), Hughes et al.
patent: 5789163 (1998-08-01), Drolet
patent: 6329145 (2001-12-01), Janjic
patent: 6670132 (2003-12-01), Janjic
patent: 2 183 661 (1987-06-01), None
patent: WO89/06694 (1989-07-01), None
patent: WO91/19813 (1991-12-01), None
patent: WO92/14843 (1992-09-01), None
patent: WO96/04557 (1996-02-01), None
Osborne et al. (1997) Current Opinion in Chemical Biology 1(1):5-9.
Tuerk (1997) Methods in Molecular Biology 67:219-230.
Tian et al. (1995) RNA 1:317-326.
Ellington & Szostak (1990) Abstracts of papers presented at the 1990 meeting on RNA Processing, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 84.
Fox et al. (1998) Drug Discovery & Development (Supplement to R& D Magazine), pp. 32-37.
Joyce (1989) Gene 82:83.
Joyce & Inoue (1989) Nucleic Acids Research 17:711.
Kinzler & Vogelstein (1989) Nucleic Acid Research 17:3645.
Kramer et al. (1974) J. Mol. Biol. 89:719.
Levisohn & Spiegelman (1969) Proc. Natl. Acad. Sci. USA 63:805.
Levisohn & Spiegelman (1968) Proc. Natl. Acad. Sci. USA 60:866.
Oliphant et al. (1989) Mol. Cell. Biol. 9:2944.
Oliphant & Struhl (1988) Nucleic Acids Research 16:7673.
Oliphant & Struhl (1987) Methods in Enzymology 155:568.
Oliphant et al. (1986) Gene 44:177.
Robertson & Joyce (1990) Nature 344:467.
Szostak, “Structure and Activity of Ribozymes,” inRedesigning the Molecules of Life, (S.A. Benner ed.) Springer-Verlag Berlin Heidelberg, pp. 87-113, (1988).
Thiesen & Bach (1990) Nucleic Acids Research 18:3203.
Gold Larry
Janjic Nebojsa
Gilead Sciences, Inc.
Naff David M.
Swanson & Bratschun L.L.C.
LandOfFree
Determining non-nucleic acid molecule binding to target by... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Determining non-nucleic acid molecule binding to target by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Determining non-nucleic acid molecule binding to target by... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3851659