Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1988-12-29
1992-10-27
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 4352402, 43524023, 435240243, 435948, C12Q 104, C12N 500
Patent
active
051588749
ABSTRACT:
The present invention discloses a biologically active basement membrane composition. When polymerized under physiogical conditions, the composition forms gel-like structures whose ultrastructure resembles interconnected thin sheets of the lamina densa zone of basement membrane. The major components of the composition include laminin, type IV collagen, heparan sulfate proteoglycan, entactin and nidogen. These components polymerize in constant proportions when redissolved and allowed to reconstitute. Molecular sieve studies on the soluble extract demonstrate that laminin, entactin and nidogen are associated in a large but dissociable complex. The reconstituted matrix is biologically active and stimulates the growth and differentiation of a variety of cells, including epithelial cells, nerve cells, hair follicles and the like. The reconstituted matrix can also be used for determining metastatic potential of tumor cells and for isolating metastatic tumor cells.
REFERENCES:
Terranova Science 226:982-984 (1984).
Martin (1984) Ciba Found. Symp. 108, 197-212.
Kleinman et al, Basement Membrane Complexes with Biological Activity, Biochemistry 25:312-318 (1986).
Albini et al, a Rapid In Vitro Assay for Quantitating the Invasive Potential of Tumor Cells, Cancer Research 47:3239-3245 (1987).
Kleinman et al., Formation of a Supramolecular Complex Is Involved in the Reconstitution of Basement Membrane Components, Biochemistry, 22:4969-4974 (1983).
Hendrix et al, Comparison of Tumor Cell Invasion Assays: Human Amnion versus Reconstituted Basement Membrane Barriers, Invasion and Metastasis 9:278-297 (1989).
Starkey, Cell-Matrix Interactions During Tumor Invasion, Cancer and Metastasis Reviews 9:113-123 (1990).
Terranova et al, Use of a Reconstituted Basement Membrane to Measure Cell Invasiveness and Select for Highly Invasive Tumor Cells, Proc. Natl. Acad. Sci. USA 83:465-469 (1986).
Kleinman Hynda K.
Martin George R.
Naff David M.
The United States of America as represented by the Department of
Williams Jane A.
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