Determining ion flux of embryos and oocytes

Details Determining ion flux of embryos and oocytes Determining ion flux of embryos and oocytes

1996-10-16

2000-05-16

Yu, Mickey

Surgery

Miscellaneous

Methods

600300, H61B 1900

Patent

active

060622253

ABSTRACT:
A non-invasive self-referencing ion-sensitive probe is used to measure ion efflux from an embryo or an oocyte to determine viability and developmental potential.

REFERENCES:
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Hush et al. "A Calcium Influx Precedes Organogenesis in Graptopetalum" Plant Cell And Environ 14(7), Sep. 1991.
Zivkovic et al. "Localized Activity of CA2+-Stimulated Atpase and Transcellular Ionic Currents During Mesoderm Induction in Embryos of Lymnaea Stagnalis (Mollusca)" Roux's Arch Dev Biol 200: 320-9, 1991.
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Smith, P.J.S., et al., Methods in Cell Biology, vol. 40, Chapter 5, The Vibrating Ca.sup.2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca.sup.2+ Influx and Efflux, pp. 115-134.
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