Determination of urea

Chemistry: analytical and immunological testing – Nitrogen containing – Urea or blood urea nitrogen

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422 61, G01N 3362

Patent

active

044748884

ABSTRACT:
A colorimetric urea determination method, reagent, and reagent kit useful in end point and kinetic urea determination is disclosed. Urea in a liquid sample reacts with o-phthalaldehyde and a chromogenic compound in the presence of a linear long hydrocarbon chain amidobetaine to produce an intensely colored reaction product, the concentration of which is linearly related to the urea concentration in the sample.

REFERENCES:
patent: 3890099 (1975-06-01), Jung
patent: 4074972 (1978-02-01), Denney
patent: 4105408 (1978-08-01), Denney
patent: 4131425 (1978-12-01), Denney
patent: 4131429 (1978-12-01), Denney
patent: 4131430 (1978-12-01), Denney
patent: 4215995 (1980-08-01), Turk
patent: 4239649 (1980-12-01), Gindler
patent: 4273556 (1981-06-01), Gindler
patent: 4357144 (1982-11-01), Gindler
Chemical Abstracts, 90: 199783u (1979).
D. Jung et al., Clin. Chem., 21 (8), 1136-1140 (1975).

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