Determination of triglycerides and glycerol

Details Determination of triglycerides and glycerol Determination of triglycerides and glycerol

1976-10-08

1979-03-06

Naff, David M.

Textiles: fiber preparation

195 99, 1951035R, C07G 702, G01N 3114

Patent

active

041429389

ABSTRACT:
Triglycerides in biological fluids containing interfering substances, such as phospholipids, are determined by hydrolyzing the triglycerides to glycerol in a short time span at moderate temperatures with a solution of alkali metal hydroxide in methanol, and treating the resultant hydrolysis mixture with a water soluble magnesium salt to precipitate the interfering substances. Glycerol is then colorimetrically determined in the resultant supernatant solution by incubating with a single reagent-substrate composition to develop a measurable color.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3713986 (1973-01-01), Bergmeyer et al.
patent: 4056442 (1977-11-01), Huang et al.
Klotzsch et al., Abstract CC31, Technicon International Congress, Technicon Instruments Corporation (1972).
Wieland, "Method of Enzymatic Analysis", by Bergmeyer, Academic Press, N. Y. & London, (1965) pp. 211-214.
Hycel Brochure on Triglyceride Test by Hycel, Inc., 1975.

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