Determination of the number of fluorescent molecules on calibrat

Details Determination of the number of fluorescent molecules on calibrat Determination of the number of fluorescent molecules on calibrat

1995-03-29

1997-04-15

Knode, Marian C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435180, 435967, 435 75, 436528, 436531, 436 10, 436800, G01N 3100

Patent

active

056208421

ABSTRACT:
A rapid, simple method for preparing beads for calibrating flow cytometers which contain a known number of fluorophores per bead is presented. Briefly, the invention utilizes beads coated with a stable complex of a fluorophore and an enzyme. the enzymatic activity of a known number of beads gives an accurate measure of fluorophore density on those beads.

REFERENCES:
patent: 4699828 (1987-10-01), Schwart et al.
patent: 5380663 (1995-01-01), Schwartz et al.
patent: 5478722 (1995-12-01), Caldwell
patent: 5494793 (1996-02-01), Schindele

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