Chemistry: analytical and immunological testing – Blood gas
Patent
1982-04-02
1984-06-12
Turk, Arnold
Chemistry: analytical and immunological testing
Blood gas
422 68, 436 66, 436133, 436163, G01N 3372, G01N 3384
Patent
active
044542291
ABSTRACT:
In order to simplify the operation for determination of the acid-base status of blood, composed of the values for pH, pCO.sub.2 and the base excess BE, the base excess BE is determined through measurement of the pH-value at a pCO.sub.2 of about 0 mm Hg. The pH-values may be determined photometrically, in which case the indicator solution for measuring the actual blood-pH is an aqueous solution of 40 micromol/l bromthymol blue plus 0.2 g/l sodium dodecylsulfate plus 1% ethanol; and for measurement of the base excess BE is an aqueous solution of 65 micro mol/l naphtholphthalein plus 0.2 g/l sodium dodecylsulfate plus 15% dimethylsulfoxide, which are measured at a wavelength of 635 nm. If desired, one may use a single solution for measuring both actual blood-pH as well as the base excess BE, composed of 16 micromol/l bromthymol blue plus 34 micromol/l naphtholphthalein plus 0.15 g/l sodium dodecylsulfate, which is measured at 615 nm wavelength.
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patent: 3899295 (1975-08-01), Halpern
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Siggaard-Andersen, "The Acid-Base Status of the Blood", 4th Edition, Williams & Wilkins Co., Baltimore, 1974, pp. 12-16 and 27.
Scribner et al., Journal of the American Medical Assoc., 155, 644-648, (1954)..
Zander et al., Chemical Abstracts, vol. 91, 1979, No. 91:206910j.
Wolf Hans U.
Zander Rolf
Striker Michael J.
Turk Arnold
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