Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2008-03-25
2008-03-25
Tsang, Cecilia J. (Department: 1654)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C436S128000
Reexamination Certificate
active
11247409
ABSTRACT:
Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d5testosterone.
REFERENCES:
patent: 5772874 (1998-06-01), Quinn et al.
patent: 5795469 (1998-08-01), Quinn et al.
patent: 5919368 (1999-07-01), Quinn et al.
patent: 5968367 (1999-10-01), Quinn et al.
patent: 6107623 (2000-08-01), Bateman et al.
patent: 6124137 (2000-09-01), Hutchens et al.
patent: 6204500 (2001-03-01), Whitehouse et al.
patent: 6268144 (2001-07-01), Koster
patent: 6410913 (2002-06-01), Brekenfeld et al.
patent: 6855703 (2005-02-01), Hill et al.
patent: 2004/0235193 (2004-11-01), Soldin
Williams et al. Electrospray Collision-induced Dissociation of Testosterone and Testosterone Hydroxy Analogs. J Mass Spectrom. 1999. vol. 34, pp. 206-216.
Carignan et al., “High-performance liquid chromatographic analysis of estradiol valerate-testosterone enanthate in oily formulations”J Chrom301(1):292-96 (1984).
Choi et al., “Rapid HPLC-Electrospray Tandem Mass Spectrometric Assay for Urinary Testosterone and Dihydrotestosterone Glucuronides from Patients with Benign Prostate Hyperplasia.” Clin. Chem. 49: 322-5 (2003).
Choi et al., “Determination of Four Anabolic Steroid Metabolites by Gas Chromatogrophy/Mass Spectrometry with Negative Ion Chemical Ionization and Tandem Mass Spectrometry”Rapid Commun. Mass Spectrom12, 1749-55 (1998).
Dorgan et al., “Measurement of steroid sex hormones in serum: a comparison of radioimmunoassay and mass spectrometry.” Steroids 67: 151-8 (2002).
Draisci et al., Quantitation of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography—tandem mass spectrometry. Journal of Chromatography, 870: 511-522, 2000.
Furuta et al., “Simultaneous Measurements of Endogenous and Deuterium-Labelled Tracer Variants of Androstenedione and Testosterone by Capillary Gas Chromatography-Mass Spectrometry.”J Chrom, Biomed Applvol. 525: 15-23, (1990).
Giraudi et al., “Effect of Tracer Binding to Serum Proteins on the Reliability of a Direct Free Testosterone Assay.” Steriods 52: 423-4 (1988).
Griffiths et al., Derivatisation for the characterization of neutral oxosteroids by electrospray and matrix-assisted laser desorption/ionization tandem mass spectrometry: the Girard P derivative. Rapid Communications in Mass Spectrometry, 17:924-935, 2003.
Lewis et al., DOT/FAA/AM-00/20 A Novel method for the determination of Sildenafil (Viagra®) and its metabolite (UK-103,320) in postmortem specimens using LC/MS/MS and LC/MS/MS/MS. National Technical Information Service, 2000, 3 cover pages and pp. 1-12.
Marcus and Durnford, “A Simple Enzyme-Linked Immunosorbent Assay for Testosterone.” Steroids 46: 975-86 (1985).
Merchant and Weinberger, “Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry.”Electrophoresis21: 1164-67 (2000).
Minut et al., “Urinary 5α-androstanediol and 5β-androstanediol measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography.”Int'l J Biol. Markers, vol. 14(3); 154-59 (1999).
Ooi and Donnelly, “More on the Analog Free-Testosterone Assay.” Clin. Chem. 45: 714-715 (1999).
Robb et al., “Atmospheric pressure photoionization: An ionization method for liquid chromatography-mass spectrometry.”Anal. Chem. 72(15): 3653-3659 (2000).
Ropero-Miller et al., Simultaneous quantitation of opioids in blood by GC-EL-MS analysis following deproteination detautomerization of keto analytes, solid-phase extraction, and trimethylsilyl derivatization. Journal of Analytical Toxicology, 26: 524-528, 2002.
B. Starcevic, et al., “Liquid Chromatography—tandem Mass Spectrometry Assay For Human Serum Testosterone And Trideuterated Testosterone,”Journal of Chromatographyl B792:197-204 (2003).
Tiller et al., Drug quantitation on a benchtop liquid chromatography-tandem mass spectrometry system. Journal of Chromatography. 771: 119-125, 1997.
Winters et al., “The analog free testosterone assay: are the results in men clinically useful?” Clin. Chem. 44:2178-2182, (1998).
Wright et al., “Proteinchip surface enhanced laser desorption/ionization (SELDI) mass spectrometry: a novel protein biochip technology for detection of prostate cancer biomarkers in complex protein mixtures.”Prostate Cancer and Prostatic Diseases2: 264-76 (1999).
Zimmer et al., “Comparison of turbulent-flow chromatography with automated solid-phase extraction in 96-well plates and liquid-liquid extraction used as plasma sample preparation techniques for liquid chromatography-tandem mass spectrometry.”J. Chromatogr. A854: 23-35 (1999).
Carns Darren A
Caulfield Michael P.
Reitz Richard E
Foley & Lardner LLP
Garcia Marcela Cordero
Quest Diagnostics Investments Incorporated
Tsang Cecilia J.
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