Determination of protein

Details Determination of protein Determination of protein

1979-02-12

1980-12-16

Gron, Teddy S.

Chemistry: physical processes

Physical processes

Crystallization

252408, 424 3, G01N 3316, C09K 300

Patent

active

042394953

ABSTRACT:
An aqueous reagent system for the quantitative determination of protein is disclosed. The system contains Coomassie Brilliant Blue G-250 dye, a monobasic strong acid having a pK.sub.a of less than 3 (e.g. HCl), a polybasic phosphonic acid (e.g. NTP), a water soluble, non-ionic surfactant characterized as being a block copolymer containing terminal polyethylene oxide segments separated by a polypropylene oxide segment. Preferably the system also contains a small amount of an alcohol such as methanol or ethanol.

REFERENCES:
patent: 3533749 (1970-10-01), Kleinman
patent: 3754865 (1973-08-01), Gindler
patent: 3873272 (1975-03-01), Wakefield
patent: 3884637 (1975-05-01), Gindler
patent: 3915643 (1975-10-01), Gindler
patent: 3947250 (1976-03-01), Pollack
patent: 4023933 (1977-05-01), Bradford et al.
Sedmak, J. J., et al., Anal. Biochem., vol. 79, pp. 544-552 (May 1977).
Miyada, D., et al., Clin. Chem., vol. 18, No. 1, pp. 52-56 (1972).
Doumas, B., et al., Clin. Chim. Acta, vol. 31, pp. 87-96 (1971).

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