Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1982-07-27
1985-01-15
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435184, C12Q 138, C12N 999
Patent
active
044938918
ABSTRACT:
A new method of determining oxidized .alpha.-1-proteinase inhibitor in serum or plasma for use in studying the development of chronic obstructive lung disease is disclosed. Levels of oxidized .alpha.-1-proteinase inhibitor indicate the potential for emphysema development with higher levels showing a decrease in lung protection against elastolytic enzymes such as elastase. This method can be used for patients with a potential for chronic obstructive lung disease rather than having to use bronchial lavage methods for such patients. No other method is known to exist for determining oxidized .alpha.-1-proteinase inhibitor in serum or plasma.
REFERENCES:
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D. C. S. Hutchison, Alpha-1-Antitrypsin Deficiency and Pulmonary Emphysema: The Role of Proteolytic Enzymes and Their Inhibitors, Brit. J. Dis. Chest 67:171, 1973.
Jack Lieberman, Involvement of Leukocytic Proteases in Emphysema and Antitrypsin Deficiency, Arch. Environ. Health 27:196, 1973.
Peter D. Kaplan, Charles Kuhn, and John A. Pierce, The Induction of Emphysema with Elastase. I. The Evolution of the Lesion and the Influence of Serum, J. Lab. Clin. Med. 82:239, 1973.
C. B. Laurell and S. Eriksson, The Electrophoretic .alpha.1-Globulin Pattern of Serum in 1-Antitrypsin Deficiency, Scandinav, J. Clin. & Lab. Invest. 15:132-134, 1963.
B. Pilacik and J. Kowalczyk, Termograficzna Proba Czynnosciowa W Diagnostyce Zmian Naczyniowych W Zespole Wibracyjnym, Med. Pr. 30(3):207, 1979.
Philip Kimbel and Friedrich Kueppers, the Biochemical Basis of Emphysema: The Oxidant Effect of Cigarette Smoke?, Annals of Internal Medicine 92(4):564, 1980.
A. Janoff, H. Carp, and D. K. Lee, Inactivation of Alpha-1-Proteinase Inhibitor and Bronchial Mucous Proteinase Inhibitor by Cigarette Smoke in vitro and in vivo, Bull. europ. Physiopath. resp. 16 (Suppl.):321, 1980.
J. E. Gadek, G. W. Hunninghake, G. A. Fells, R. L. Zimmerman, B. A. Keogh, and R. G. Crystal, Evaluation of the Protease-Antiprotease Theory of Human Destructive Lung Disease, Bull. europ. Physiopath. resp. 16 (Suppl.):27, 1980.
J. Travis, K. Beatty, P. S. Wong, and N. R. Matheson, Oxidation of Alpha-1-Proteinase Inhibitor as a Major, Contributing Factor in the Development of Pulmonary Emphysema, Bull. europ. Physiopath. resp. 16 (Suppl.):341, 1980.
G. D. Billingsly, and D. W. Cox, Functional Assay of .alpha.1-Antitrypsin in Obstructive Lung Disease, Am. Rev. resp. Disease 121 (1):161-164, 1980.
G. M. Turino, R. M. Senior, D. C. Bhagwin, S. Keller, M. M. Levi, and I. Mandl, Serum Elastase Inhibitor Deficiency and .alpha.1-Antitrypsin Deficiency in Patients with Obstructive Emphysema, Science 165:709, 1969.
D. C. S. Hutchison, Alpha-1-Antitrypsin Deficiency and Pulmonary Emphysema: The Role of Proteolytic Enzymes and Their Inhibitors, Brit. J. Dis. Chest 67:171, 1973.
Jack Lieberman, Involvement of Leukocytic Proteases in Emphysema and Antitrypsin Deficiency, Arch. Environ. Health 27:196, 1973.
Peter D. Kaplan, Charles Kuhn, and John A. Pierce, The Induction of Emphysema with Elastase. I. The Evolution of the Lesion and the Influence of Serum, J. Lab. Clin. Med. 82:239, (1973).
C. B. Laurell and S. Eriksson, The Electrophoretic .alpha.1-Globulin Pattern of Serum in 1-Antitrypsin Deficiency, Scandinav. J. Clin. & Lab. Invest. 15:132-134, 1963.
B. Pilacik and J. Kowalczyk, Termograficzna Proba Czynnosciowa W Diagnostyce Zmian Naczyniowych W Zespole Wibracyjnym, Med. Pr. 30(3):207, 1979.
Philip Kimbel and Friedrich Kueppers, The Biochemical Basis of Emphysema: the Oxidant Effect of Cigarette Smoke?, Annals of Internal Medicine 92(4):564, 1980.
A. Janoff, H. Carp, and D. K. Lee, Inactivation of Alpha-1-Pr
The Government has rights in this invention pursuant to grant No. HL26148 awarded by the Department of Health and Human Services.
Kepplinger Esther M.
University of Georgia Research Foundation Inc.
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