Determination of microbial cells in aqueous samples

Radiant energy – Radiation tracer methods

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356 39, 424 3, 424 7, 424 8, 424 12, 435 7, 435 29, 23230B, C12Q 129, G01N 2100, G01N 3348

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042257832

ABSTRACT:
The present invention encompasses a method for enumerating microbial cells in an aqueous test sample comprising: fluorescently labeling the microbial cells in test sample intermixing a known amount of monodispersed fluorescently labeled beads of about cell size into the test sample, and determining the ratio of bacterial cells to fluorescently labeled beads, the reagents for practicing this method. The methods and reagents of the present invention are particularly useful in counting bacterial cells in urine.

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