Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1990-08-24
1993-12-28
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 23, 435 681, 435193, 436161, 436 86, 514 45, 514 46, 530300, 530335, 530344, 530345, 930 20, 930 22, C12Q 148
Patent
active
052738868
ABSTRACT:
The invention relates to methods and means for quantitative determination of the isoaspartyl content of polypeptides via selective methylation of their fragments, catalyzed by a protein L-isoaspartyl methyltransferase enzyme. Since deamidation of asparagine side chains at specific sites of proteins and the resultant isoaspartate formation are emerging as a major contributor to protein degradation under mild conditions, the invention also concerns a method for quantitation of protein degradation associated with isoaspartate formation.
REFERENCES:
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This invention was made with Government support under Grant Nos. 5-RO1-NS17269, 5-PO1-AG00538, and 5-KO4-NSO1082 awarded by the National Institute of Health. The Government has certain rights on this invention.
Griffin Ronald W.
The Reagents of The University of California
Webber Pamela S.
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