Determination of ions in fluids

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 4, 435 18, 435 25, 435962, C12Q 100, C12Q 126, C12Q 134, C12Q 140

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active

054098149

ABSTRACT:
A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.

REFERENCES:
patent: 4299916 (1981-11-01), Litman
patent: 4657854 (1987-04-01), Wegfahrt
patent: 4677069 (1987-06-01), Chen
Outlaw William, Measurement of Potassium by . . . Anal Biochem 92, 370-374 1979.
Wimmer, Michael, A Kinetic Colorimetric Procedure . . . Clin Chem 32, 4, 629-632 1986.

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