Determination of intracellular antioxidant levels

Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Glycoproteins

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

436 63, 436 86, 436 93, 436 99, 436161, 436813, 435 2, G01N 3300

Patent

active

06020205&

ABSTRACT:
A method of determining intracellular levels of water-soluble and fat-soluble antioxidants. Peripheral blood mononuclear cell lysates are prepared from an individual and analyzed by HPLC, either directly or after extraction with organic solvents to extract fat-soluble antioxidants. The levels of antioxidants are compared to normal levels as an indication of the overall oxidative health of the individual.

REFERENCES:
patent: 5607974 (1997-03-01), Droge et al.
patent: 5830455 (1998-11-01), Valtuena et al.
patent: 5843785 (1998-12-01), Herzenberg et al.
Eck et al. Biological Chemistry--Hoppe--Seyler, vol. 370, pp. 101-108, Feb. 1989.
Fotouhi et al. American Journal of Clinical Nutrition, vol. 63, pp. 553-558, 1996.
Baker et alt., Our Experiences with Vitamin Malabsorption: An Overview, Surv. Dig. Dis. 1:203-216 (1983).
Block, Gladys, Vitamin C and cancer prevention: the epidemiologic evidence, Am. J. Clin. Nutr. 53:270S-282S (1991).
Levine, et al., Vitamin C pharmacokinetics in healthy volunteers: Evidence for a recommended dietary allowance, Proc. Natl. Acad. Sci. USA, 93:3704-3709 (1996).
Lykkesfeldt, et al., Determination of Ascorbic Acid and Dehydroascorbic Acid in Plasma by High-Performance Liquid Chromatorgraphy with Coulometric Detection--Are They Reliable Biomarkers of Oxidative Stress, Analytical Biochemistry, 229:329-335 (1995).
Patterson, et al., Vitamin supplements and cancer risk: the epidemiologic evidence, Cancer Causes and Control, 8:786-802 (1997).
Rose, et al, Analysis of water-soluble antioxidants by high-pressure liquid chromatography, Biochem. J. 306:101-105 (1995).
Shamberger, Raymond J., Antioxidants and Cancer, vol. II 53-58 (1983).
Sinha et al., Serum Ascorbic Acid Stability Over An Extended Period: Relevance to Epidemiological Studies; Nutrition Research, 17(9)1409-1415 (1997).
Stahl, et al., Cis-trans Isomers of Lycopene and .beta.-Carotene in Human Serum and Tissues, Arch. Biochem. Biophys. 294(1):173-177 1992).
Zhu, et al., Vitamin E concentration in breast adipose tissue of breast cancer patients (Kuopio, Finland), Cancer Causes and Control 7:591-595 (1996).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Determination of intracellular antioxidant levels does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Determination of intracellular antioxidant levels, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Determination of intracellular antioxidant levels will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-936811

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.