Determination of hydrogen peroxide

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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23230B, 252408, 435 10, 435 11, 435 12, 435 14, 435 20, C12Q 128, G01N 3100

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active

042516293

ABSTRACT:
Colorimetrical determination of hydrogen peroxide in clinical examinations, food additives analyses and general analyses can be carried out accurately, rapidly and economically by using a composition for producing color producing reagents comprising 4-aminoantipyrine, a N-substituted-3-alkylaniline and a hydrogen peroxide activating agent such as peroxidase, said composition forming a coloring material having excellent sensitivity and color stability in contact with hydrogen peroxide.

REFERENCES:
patent: 3886045 (1975-05-01), Meiattini
patent: 3979262 (1976-09-01), Hunziker
Kabasakalian et al., "Enzymatic Blood Glucose Determination by Colorimetry of N,N-Diethylaniline-4-Aminoantipyrine, Clin. Chem., vol. 20, No. 5, (1974), pp. 606-607.
Takayama et al., "A New Enzymatic Method for Determination of Serum Cholin-Containing Phospholipids," Clin. Chem. Acta, vol. 79, (1977), pp. 93-98.
Eisenstaedt, "The Condensation of Aminoantipyrine with Aromatic Amines in the Presence of Oxidizing Agents," J. Org. Chem., vol. 3, (1939), pp. 153-165.

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