Determination of high molecular weight analytes using a .beta.-g

Details Determination of high molecular weight analytes using a .beta.-g Determination of high molecular weight analytes using a .beta.-g

1991-02-01

1993-09-14

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 76, 435 78, 435 18, 435975, 436501, 436537, G01N 33542, G01N 33566, G01N 33569

Patent

active

052447855

ABSTRACT:
A method for detecting the presence of a high-molecular-weight analyte, which uses a variation on complementation assays, comprising (1) providing an enzyme donor (ED)complex comprising ED coupled to an analyte-specific binding molecule, wherein the ED complex retains measurable complementation activity such that active .beta.-galactosidase is formed in the presence of an enzyme acceptor (EA); (2) contacting the ED complex and EA in the presence of a sample suspected of containing a high-molecular-weight analyte that reacts specifically with the analyte-specific binding molecule; and (3) relating the presence of the analyte in the sample to the formation of active .beta.-galactosidase enzyme.

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