Determination of HDL whole blood

Details Determination of HDL whole blood Determination of HDL whole blood

1989-11-17

1992-06-02

Lacey, David L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

436 71, 436 86, 436 87, 436177, 436179, G01N 3392, G01N 2131, G01N 2177, G01N 3349

Patent

active

051186136

ABSTRACT:
A method for separating HDL from whole blood anticoagulated with EDTA is disclosed. The method includes, as a first step, mixing the anticoagulated whole blood with an aqueous solution composed of magnesium .sup.++ cations and dextran sulfate 500 in such proportions and of such concentration

REFERENCES:
patent: 4699887 (1987-10-01), Abbott et al.
patent: 4772561 (1988-09-01), Genshaw
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Bentzen et al., "Direct Determination of Lipoprotein Cholesterol Distribution With Micro-Scale Affinity Chromatrography Columns", Clinical Chemistry, vol. 28, No. 7, 1451-1456 (1982).
Lippi et al., "Determination of High Density Lipoprotein Cholesterol In Venous And Capillary Whole Blood", Journal Of Lipid Research, vol. 29, 112-115 (1988).
Abbott Laboratories, Abbott Park, Illinois, Product Literature, "Vision.RTM. Whole Blood HDL Cholesterol" Pamphlet No. 3A21.
Burstein et al. "Rapid Method For The Isolation of Lipoproteins From Human Serum By Precipitation With Polyanions", Journal Of Lipid Research, vol. 11, 583-59 (1970).
Finley et al., "Cholesterol in High-Density Lipoprotein: Use of Mg.sup.2+ /Dextran Sulfate In Its Enzymatic Measurement" Clinical Chemistry, vol. 24, No. 6, 931-933 (1978).
C. Allain et al., "Enzymatic Determination of Total Serum Cholesterol", Clinical Chemistry, vol. 20, No. 4, 470-476 (1974).
Artiss et al., "A Sensitive Reaction for Dilute Cholesterol Determinations", Michrochemical Journal, 25, 535-542 (1980).

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