Determination of genomic sex in salmonids

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 435270, 435810, 536 231, 536 2351, 536 2431, 536 2433, 935 9, 935 76, 935 77, 935 80, C12Q 168, C12P 1934, C07H 2104, C12N 1500

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054807743

ABSTRACT:
The invention provides a method for determining the genomic sex of various salmonids (family salmonidae). In particular, the invention provides the nucleic acid sequence of a pseudogene, designated GH-.PSI., which is linked to a sex determining locus on the Y chromosome, and may be used as a marker for determination of the sex of the fish.

REFERENCES:
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Devlin et al., Can. J. Fish Aquat. Sci., vol. 48, pp. 1606-1612, 1991.
Du et al., I.U.B.S. Toronto Symposium on "Advances in the Molecular Endocrinology of Fish," May 23-25 (1993).
T. Maniatis et al., Molecular Cloning, a laboratory manual, published 1982 by McGraw-Hill (N.Y.), pp. 280-281.
Bio/Technology, vol. 3, issued Nov. 1985, Saiki et al., "A novel method for the detection of polymorphic restirction sites by cleavage of oligonucleotide probes: Application to sickle-cell anemia," pp. 1008-1012.
Nature, vol. 318, No. 6046, issued 12-18 Dec. 1985, Gill et al., "Forensic application of DNA `fingerprints`", pp. 577-579.
Nature, vol. 335, No. 6189, issued 29 Sep. 1988, Li et al., "Amplification and analysis of DNA sequences in single human sperm and diploid cells," pp. 414-417.

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