Determination of concentration by affinity titration

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 793, 435810, 435970, 435300, 435301, G01N 3353, G01N 33543

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active

053567846

ABSTRACT:
A method for determining the amount of analyte in a sample utilizes a series of test regions with systematically varied, preferably monotonically increasing, affinity for a specific binding partner for an analyte or for the analyte itself. By determining the portion of test regions which are capable of binding specific binding partner in competition with the analyte in the sample, or which bind the analyte, the amount of analyte may be estimated. Thus, titration of affinity replaces titration of concentration, allowing assays to, be performed without the need for serial dilution steps.

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Practice and Theory of Immunoassays, Elsevier Science Publishers, Amsterdam, The Netherlands, pp. 126-132, 1985.

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