Determination of chlamydia trachomatis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 21, 435 25, 435 26, 435 29, 436518, 436825, G01N 3353, G01N 33543, C12Q 142

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active

048309605

ABSTRACT:
A process for determining qualitatively or quantitatively the presence of Chlamydia Trachomatis or Chlamydia trachomatis derived material in a subject material which process comprises deriving a sample from said subject material Chlamydia trachomatis derived material, contacting said sample with an antibody for a Chlamydia trachomatis derived material bound to a substrate thereby to bind at least a portion of the Chlamydia trachomatis derived material, contacting the bound Chlamydia trachomatis derived material with a conjugate between a substance which will bind selectively to the Chlamydia trachomatis derived material and a catalyst for a reaction system thereby to bind said conjugate to said bound Chlamydia trachomatis derived material, carrying out the reaction system catalysed by said catalyst and determining a product of said reaction system, characterised in that the sample is derived from the subject material by a process including heating the subject material to an elevated temperature and for a period of time such as to enhance the determination.

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