Determination of cells in blood

Details Determination of cells in blood Determination of cells in blood

1977-03-04

1978-06-27

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

195 1, G01N 2102, G01N 3102, G01N 3122, G01N 3316

Patent

active

040972372

ABSTRACT:
A method for determining a volume of red cells in a blood sample. The method includes the steps of flowing along a conduit a predetermined volume of the sample, introducing an agglutinating reagent and a predetermined volume of a dye into the conduit to mix with the sample and agglutinate the red cells, the dye being of known concentration on the introduction thereof, decanting the agglutinated red cells from the conduit, and colorimetrically measuring the concentration of the dye in the conduit, the agglutinating reagent being characterized by a relatively high positive electrical charge and the dye being water soluble and characterized by not penetrating said cells and not forming a precipitate with the agglutinating reagent or with the soluble components of the sample and not lysing the cells or altering the volume of the cells.

REFERENCES:
patent: 3334018 (1967-08-01), Smythe
patent: 3427135 (1969-02-01), Pelavin et al.
patent: 3627495 (1971-12-01), Adler, Jr. et al.
patent: 3648160 (1972-03-01), Beaver
patent: 3684450 (1972-08-01), Adler et al.
patent: 3902964 (1975-09-01), Greenspan
Hemolysis and Related Phenomena; Eric Ponder, Grune & Stratton, New York 1948, pp. 51-53.
A New Method for the Detection of Red Cell Antitbodies, Parviz Allezari, Automation in Analytical Chemistry, Technicon Symposium, vol. 1, 1967.

Affiliated with

Also associated with

Rating

Determination of cells in blood does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Determination of cells in blood, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Determination of cells in blood will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2302068