Determination of .beta.-lipoproteins in blood serum with polyane

Details Determination of .beta.-lipoproteins in blood serum with polyane Determination of .beta.-lipoproteins in blood serum with polyane

1976-10-08

1978-08-29

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

260112B, G01N 3316, G01N 3102, G01N 2122

Patent

active

041100778

ABSTRACT:
A process of isolating beta-lipoproteins from blood serum in about 99% purity comprising precipitating beta-lipoproteins from blood serum substantially free of chylomicrons with polyanethole sulfonate and a divalent cation, forming a solution of the beta-lipoproteins and reprecipitating them with the divalent cation, again forming a solution of said beta-lipoproteins and passing them through a DEAE cellulose column. The beta-lipoproteins purified by the method are particularly suited as a calibration standard for use in assays of the beta-lipoprotein content of blood serum.

REFERENCES:
patent: 3894844 (1975-07-01), Pinto
patent: 3954409 (1976-05-01), Hsia
patent: 3955925 (1976-05-01), Proksch
patent: 3958939 (1976-05-01), Jones
patent: 3958939 (1976-05-01), Jones
patent: 3960493 (1976-06-01), Beitz
patent: 3993585 (1976-11-01), Pinto
patent: 4001089 (1977-01-01), Stavropoulos
patent: 4011045 (1977-03-01), Bonderman
patent: 4012196 (1977-03-01), Frings
patent: 4012287 (1977-03-01), Carl
patent: 4045176 (1977-08-01), Proksch
Chemical Abstracts, 73:118082p (1970).
Chemical Abstracts, 75:149579b (1971).
M. Burstein et al., Adv. Lipid Res., 11, 67-108 (1973).

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