Determination of analytes in biological fluids in the presence o

Details Determination of analytes in biological fluids in the presence o Determination of analytes in biological fluids in the presence o

1995-02-23

2000-01-04

Chin, Christopher L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 57, 422 58, 4352871, 4352872, 435810, 435962, 435973, 436 71, 436175, 436178, 436177, 436518, 436528, 436539, 436541, 436531, 436824, 436825, G01N 33537, G01N 33538, G01N 33543

Patent

active

060108665

ABSTRACT:
A method is provided, in one embodiment, for the determination of an analyte in a biological fluid sample in the presence of a substance interfering with an assay for the analyte. This embodiment is implemented by using antibodies to cause the selective immunoreaction of at least one of the analyte or the interfering substance and then conducting an assay for the analyte in at least one of the immunoreactants or the non-reactants. Another embodiment provides a disposable reaction device to implement the method. The invention is applicable to the detection of a wide variety of analytes, including cholesterol in a targeted lipoprotein class in the presence of cholesterol in another class; to targeted isozymes of enzymes such as creatine kinase, lactate dehydrogenase, amylase, and alkaline or acid phosphatases in the presence of other isozymes; as well as to targeted immunoglobulins in the presence of non-targeted immunoglobulins.

REFERENCES:
patent: 3932221 (1976-01-01), Pfleiderer
patent: 4236893 (1980-12-01), Rice
patent: 4244694 (1981-01-01), Farina et al.
patent: 4746605 (1988-05-01), Kerscher et al.

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