Determination of analyte concentration based on a micellar recog

Details Determination of analyte concentration based on a micellar recog Determination of analyte concentration based on a micellar recog

1995-10-24

1999-07-27

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 5, 435 721, 435 732, 435 74, 435969, 436514, 436528, 436535, 436829, 436 71, G01N 33543

Patent

active

059289570

ABSTRACT:
Starting from two non-miscible phases, i.e., a lipophilic and a hydrophilic phase, a surface-active substance and a recognition component, a micellar recognition system is built in a suitable manner and is incorporated into a layer structure. The micellar recognition system is provided in a single thin layer in which the recognition component is distributed homogeneously. Additional layers which may be provided about this layer, have an influence on the properties of the entire layer structure. On contact between the layer structure and the substance to be measured a recognition step takes place in the layer which is followed by a transducing step. In this way a measurement variable is produced by means of which information is obtained on the substance. The layer structures exhibit higher sensitivity, a lower detection threshold, short response time and special robustness with regard to sample interference.

REFERENCES:
patent: 4939098 (1990-07-01), Suzuki et al.
A.V. Kabanov et al., "A New Way in Homogeneous Immunoassay: Micellar Systems as a Medium for Analysis" in Anal. Biochem. 181, 145-148 (1989).
K. Martinek et al., "Micellar Enzymology" in Eur. J. Biochem. 155, No. 3, 453-468 (1986).

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