Determination of amylase

Details Determination of amylase Determination of amylase

1988-09-21

1990-03-27

Griffin, Ronald W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 25, 435810, 435 99, 435 22, 536 187, 536 142, C12P 1904, C12Q 126, C07H 504

Patent

active

049120417

ABSTRACT:
A method of assay for the presence of .alpha.-amylase in a sample employs a derivatized .alpha.-oligosaccharide which can be hydrolyzed by an enzyme mix including .alpha.-amylase to yield an electroactive compound, and at least one other enzyme which with .alpha.-amylase gives an enzyme mix capable of hydrolyzing the derivatized .alpha.-oligosaccharide. The sample is contacted with the derivatized .alpha.-oligosaccharide and with the other enzyme to permit hydrolysis of the derivatized .alpha.-oligosaccharide and to allow formation of the electroactive compound. An electrode is poised at a potential at which the derivatized .alpha.-oligosaccharide is not electochemically active, the potential being one at which the electroactive compound is electochemically active. Any current arising from electrochemical activity of the electroactive compound is detected.

REFERENCES:
patent: 4649108 (1987-03-01), Blair
patent: 4681841 (1987-07-01), Matsumoto et al.
patent: 4774192 (1988-09-01), Terminiello et al.
patent: 4794078 (1988-12-01), Blair

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