Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2008-09-23
2010-02-09
Cook, Lisa V (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C436S086000, C436S161000, C436S501000, C436S507000, C436S518000
Reexamination Certificate
active
07659081
ABSTRACT:
The present invention provides methods for the isolation, identification, and purification of adrenomedullin (AM)-binding proteins. Also, provided are methods for utilizing the purified AM-binding proteins, or functional portions thereof, to diagnose, treat, and monitor AM-related diseases, for example, diseases or disorders associated with abnormally elevated AM levels. In addition, the present invention provides a newly identified complex between AM and a specific AM-binding protein 1 (AMBP-1); which has been isolated and identified herein as factor H (fH). The invention also provides AM/AMBP complexes, particularly AM/FH complexes, and antibodies specifically reactive with this complexes. Further provided are methods for identifying and purifying complexes of AM and an AM binding protein using anti-AM/fH antibodies, and methods for treating conditions such as cancer or diabetes utilizing compositions comprising these antibodies. The present invention additionally provides methods for identifying antagonists agents that inhibit the function of AM, factor H, or the AM/factor H complex. The invention also provides methods for treating conditions such as cancer or diabetes using these antagonist agents.
REFERENCES:
patent: 4444879 (1984-04-01), Foster et al.
patent: 4774323 (1988-09-01), Newman et al.
patent: 4883784 (1989-11-01), Kaneko
patent: 5641685 (1997-06-01), Anumula
patent: WO 97/07214 (1997-02-01), None
patent: WO97 07214 (1997-02-01), None
Kato et al. (Endocrinology, 1997, vol. 138, No. 6, pp. 2615-2620).
Sato et al. (Life Sciences, vol. 57., No. 2, pp. 189-194, 1995).
Martinez et al. (Endocrinology, vol. 138, No. 12, 1997, pp. 5597-5604).
Congote, “Reversed-phase high pressure liquid chromatography of globin chains: its application for the prenatal diagnosis of beta-thalassemia.”Prog Clin Biol Res. 60:39-52, 1981.
Elsasser et al., “Adrenomedullin Binding Protein in the Plasma of Multiple Species: Characterization by Radioligand Blotting,”Endocrinology, 140(10):4908-4911, 1999.
Hatefi and Hanstein, “Solubilization of particulate proteins and nonelectrolytes by chaotropic agents,”Proc. Natl. Acad. Sci., 62(4):1129-36, 1969.
Kato et al., Adrenomedullin As An Autocrine/Paracrine Apoptosis Survival Factor For Rat Endothelial Cells,Endocrinology, 138(6): 2615-2620, 1997.
Martinez et al., “Adrenomedullin Binding Protein-1 (AMBP-1) Modulates Tumor Growth by Reducing Availability of Adrenomedullin (AM),”Proceedings of the American Association for Cancer Research, 41: 153, 2000.
Martinez et al., “Expression of Adrenomedullin and Its Receptor in Normal and Malignant Human Skin: A Potential Pluripotent Role in the Integument,”Endocrinology, 138(12): 5597-5604, 1997.
Martinez et al., “Is Adrenomedullin A Causal Agent In Some Cases Of Type 2 Diabetes?,”Peptides, 20: 1471-1478, 1999.
Martinez et al., “Regulation of Insulin Secretion and Blood Glucose Metabolism by Adrenomedullin,”Endocrinology, 137(6): 2626-2632, 1996.
Opperman et al., “Quantitation of Components of the Alternative Pathway of Complement (APC) by Enzyme-linked Immunosorbent Assays,”J. Immunol. Methods., 133(2): 181-190, 1990 (abstract only).
Pio et al., “Detection of Adrenomedullin Binding Proteins in Human Plasma by a Novel Non-Radioactive Method,”1999 Summer Neuropeptide Conference(Ninth Annual), Jun. 27, 1999.
Pio et al., “Isolation and Characterization of a Human Plasma Adrenomedullin Binding Protein, Analysis of Its Expression in Human Tumor Cell Lines,”Proceedings of the American Association for Cancer Research, 41: 152, 2000.
Sato et al., “Characterization Of Immunoreactive Adrenomedullin In Human Plasma And Urine,”Life Sciences, 57(2): 189-194, 1995.
Tsang et al., “Optimum Dissociating Condition For Immunoaffinity And Preferential Isolation Of Antibodies With High Specific Activity,”Journal of Immunological Methods, 138: 291-299, 1991.
Wikipedia, “chaotropic agent”, http//en.wikipedia.org/wiki/Chaotropic—agent, dated Mar. 27, 2006.
Cuttitta Frank
Elsasser Ted H.
Martinez Alfredo
Pio Rubén
Cook Lisa V
Klarquist & Sparkman, LLP
The United States of America as represented by the Secretary of
LandOfFree
Determination of AM-binding proteins and the association of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Determination of AM-binding proteins and the association of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Determination of AM-binding proteins and the association of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4156556