Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1983-08-29
1985-12-03
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
204403, 435177, 435178, 435179, 435180, 435182, 435817, 435938, 436131, 436132, C12Q 126, C12N 1102, C12R 184
Patent
active
045566356
ABSTRACT:
The concentration of a C.sub.1 to C.sub.4 alcohol in an essentially water immiscible organic system is accurately determined by a simple and quick process. A sample of the water immiscible organic system containing less than about 0.01% (W/V) of the alcohol is contacted with an aqueous layer of alcohol oxidase immobilized on an electrode for measuring dissolved oxygen content. Ethanol in the water immiscible organic system partitions into the aqueous layer where alcohol oxidase catalyzes oxidation of the alcohol. Oxygen consumed during the oxidation is measured by the electrode, and the concentration of alcohol determined therefrom. To obtain less than about 0.01% alcohol in the sample, the sample may be diluted with the water immiscible organic system.
REFERENCES:
patent: 4073713 (1978-02-01), Newman
patent: 4119494 (1978-10-01), Durand et al.
Butler, "Enzymes in Non-Aqueous Solvents", Enzyme Microb. Technol., 1, pp. 253-259, (1979).
Guilbault et al., "Amperometric Enzyme Electrodes Part III, Alcohol Oxidase", Analytica Chemica Acta, 69, pp. 189-194, (1974).
Klibanov et al., "A New Approach to Preparative Enzymatic Synthesis", Biotechnology and Bioengineering, XIX pp. 1351-1361, (1977).
Clark, Jr., Biotechnol. & Bioeng. Symp. No. 3, 1972, pp. 377-394, (John Wiley & Sons, Inc.).
Couderc, et. al., Agric. Biol. Chem. vol. 44, No. 10, 1980, pp. 2279-2289.
Hitzman Donald O.
Hopkins Thomas R.
Naff David M.
Phillips Petroleum Company
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