Determination of adenosine by immunoassay involving acylation of

Details Determination of adenosine by immunoassay involving acylation of Determination of adenosine by immunoassay involving acylation of

1982-07-09

1984-10-23

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436527, 436529, 436530, 436532, 436533, 436538, 436542, 436543, 436547, 436815, G01N 3354

Patent

active

044789343

ABSTRACT:
A method of quantative determination of adenosine by means, of competitive immunoassay based on a competitive antigen-antibody reaction. In the competitive antigen-antibody reaction, an antibody is used which is obtained from an animal which has been immunized by introduction thereto of an antigen which comprises a carrier protein bonded with 2'- and 3'-hydroxyls of the adenosine through dicarboxylic acid residues, and a labelled adenosine and 2',3'-diacyladenosine which has been produced by acylation of adenosine in the sample to be assayed or in a standard solution are caused to undergo competitive reaction for the antibody whereby it has been made possible to determine adenosine quantitatively in high sensitivity and in high accuracy.

REFERENCES:
Chemical Abstracts, 96: 177182u, (May 24, 1982).

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