Determination of a genotype of an amplification product at multi

Details Determination of a genotype of an amplification product at multi Determination of a genotype of an amplification product at multi

1998-02-04

1999-10-05

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 9121, 435 915, 536 243, C12Q 168, C12P 1934, C07H 2104

Patent

active

059622334

ABSTRACT:
A method is provided for genotyping a target sequence at at least two allelic sites by a 5' nuclease amplification reaction. In one embodiment, the method includes performing a nucleic acid amplification on a target sequence having at least two different allelic sites using a nucleic acid polymerase having 5'.fwdarw.3' nuclease activity and a primer capable of hybridizing to the target sequence in the presence of two or more sets of allelic oligonucleotide probes wherein:

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Faas et al, "Sequence specific priming and exonuclease released fluorescence detection of HLA-DQB1 alleles", Tissue Antigens 48:97-112, 1996.
Luedeck et al, "Fluorotyping of HLA-C: differential detection of amplicons by sequence specific priming and fluorogentic probing", Tissue Antigens 50:627-638, Dec. 1997.

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