Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2005-03-15
2005-03-15
Leary, Louise N. (Department: 1654)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S026000, C435S025000, C435S028000, C435S975000
Reexamination Certificate
active
06867012
ABSTRACT:
The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, and homocysteine, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteine.
REFERENCES:
patent: 5668173 (1997-09-01), Garrow
patent: 5998191 (1999-12-01), Tan et al.
patent: 0 437 373 (1991-07-01), None
patent: 0 477 001 (1992-03-01), None
patent: P2001-17198 (2001-01-01), None
patent: WO 9947559 (1999-09-01), None
patent: WO 0028071 (2000-05-01), None
van Dijken et al (Archives of microbiol, V.111(1-2),pp 77-83,(Dec. 1976)(Abstract Only).*
Mori et al., “Purification and Properties of Dimethylglycine Oxidase from Cylindrocarpon didymum M-1”, Agricultural and Biological Chemistry, 44(6):1383-1390 (1980).
Meskys, R. et al., “Peculiarities of creatinline catabolism in some Arthrobacter strains”, Biologija, 1:61-64 (1997).
Kerr R. G. et al., “An Enzyme-Based Formaldehyde Assay and Its Utility in a Sponge Sterol Biosynthetic Pathway”, J. Nat. Prod., 62(1):201-202 (1999).
Kiba N. et al., “Determination of nano-molar levels of formaldehyde in drinking water using flow-injection system with immobilized formaldehyde dehydrogenase after off-line solid-phase extraction”, Analytica Chimica ACTA, 378(1-3):169-175 (1999).
Van Ophem P. W. et al., “NAD-and co-substrate(GSH or factor)-dependent formaldehyde dehydrogenases from methylotrophic microorganisms act as a class III alcohol dehyrogenase”, FEMS Microbiology Letters, 116(1):87-93 (1994).
Kato N. et al., “Formaldehyde Dehyrdogenase from Methylotrophic Yeasts”, Methods in Enzymology, 188:455-459 (1990).
M.S. Quesenberry, et al., “A Rapid Formaldehyde Assay Using Purpald Reagent: Application under Periodation Conditions”,Anal. Biochem., 234(1), 50-55 (1996).
Kensa to Gijutsu, 27(8): 973-980 (1999).
Enzyme Handbook 9, 1.1.1.1, “Alcohol Dehydrogenase” Springer-Verlag, pp. 1-23, (1995).
Masayoshi Yasuhara, et al., “A New Enzymatic Method to Determine Creatine”,Clin. Clim. Acta, 122, 181-188 (1982).
O. Sugita, et al., “Reference values of serum and urine creatine, and of creatinine clearance by a new enzymatic method”,Ann. Clin. Biochem, 29, 523-528 (1992).
Anders Andersson, et al., “Homocysteine and Other Thiols Determined in Plasma by HPLC and Thiol-Specific Postcolumn Derivatization”,Clin. Chem., 39, 1590-1597, (1993).
Lasse Uotila, et al., “A Steady-State-Kinetic Model for Formaldehyde Dehydrogenase from Human Liver”,Biochem. J., 177, 869-878, (1979).
Bernard Vinet,“An Enzymic Assay for the Specific Determination of Methanol in Serum”,Clin. Chem., 33(12), 2204-2208 (1987).
Yuzo Kayamori, et al., “A Sensitive Determination of Uric Acid in Serum Using Uricase/Catalase/Formaldehyde Dehydrogenase Coupled with Formate Dehydrogenase”,Clin. Biochem., 27(2), 93-97 (1994).
Yutaka Teranishi, et al., “Catalase Activities of Hydrocarbon-utilizingCandidaYeasts”Agric. Biol. Chem. 38(6), 1213-1220 (1974).
Jacob W. Dubnoff, et al., “Dimethylthetin and Dimethyl-β-Propiothetin in Methionine Synthesis”,J. Biol. Chem., 176, 789-796 (1948).
Raymond F. White, et al., “Betaine-Homocysteine Transmethylase inPseudomonas denitrificans, a Vitamin B12Overproducer”,J. Bacteriol., 113(1), 218-223, (1973).
Timothy A. Garrow, “Purification, Kinetic Properties, and cDNA Cloning of Mammaliam Betaine-Homocysteine Methyltransferase”J. Biol. Chem., 271(37), 22831-22838 (1996).
Ingo Neben, et al., “Studies on an Enzyme, S-Formylglutathione Hydrolase, of the Dissimilatory Pathway of Methanol inCandida Boidinii”, Biochlm. Biophys. Acta, 614, 81-91(1980).
Horst Schütte, et al., “Purification and Properties of Formaldehyde Dehydrogenase and Formate Dehydrogenase from Candida Boidinii”,Eur. J. Biochem. 62(1), 151-150 (1976).
Hattori Shizuo
Kawamura Yoshihisa
Kishimoto Takahide
Oka Masanori
Sogabe Atsushi
Kenyon & Kenyon
Leary Louise N.
Toyo Boseki Kabushiki Kaisha
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