Detergent enzymes

Details Detergent enzymes Detergent enzymes

1994-02-08

1995-11-21

Lieberman, Paul

Compositions

Water-softening or purifying or scale-inhibiting agents

252DIG16, 455223, 455224, 455225, C11D 3386

Patent

active

054684161

DESCRIPTION:

BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATION

This application is a continuation of PCT/DK92/00273 filed Sep. 11, 1992, which is incorporated herein by reference.


TECHNICAL FIELD

This invention is in the field of detergent enzymes. More specifically, the invention relates to the use of proteases derived from fungi of the genus Verticillium for detergent purposes.
1. Background Art
Fungi belonging to the genus Verticillium are well known in the literature, and they are known to be pathogenic to insects and plants. The fungi are also known to produce proteolytic enzymes which have been investigated in relation to the pathogenicity of the fungi.
2. Summary of the Invention
It has now surprisingly been found that proteases derived from members of the fungi Verticillium posses excellent washing performance.
Accordingly, the present invention provides detergent compositions comprising proteases obtainable from, or proteases having immunochemical properties identical or partially identical to those of a protease derived from any of the strains CBS No. 145.70; CBS No. 146.70; CBS No. 247.68; and CBS No. 464.88.
In another aspect, the invention provides detergent compositions comprising proteases obtainable from a member of the genus Verticillium, or a mutant or a variant thereof.
In yet another aspect, the invention provides detergent additives comprising proteases obtainable from, or proteases having immunochemical properties identical or partially identical to those of a protease derived from any of the strains CBS No. 145.70; CBS No. 146.70; CBS No. 247.68; and CBS No. 464.88.
In a further aspect, the invention provides detergent additives comprising proteases obtainable from a member of the genus Verticillium, or a mutant or a variant thereof.


BRIEF DESCRIPTION OF DRAWINGS

The present invention is further illustrated by reference to the accompanying drawings, in which:
FIG. 1 shows the relation between temperature (.degree.C.) and proteolytic activity (% relative) of an enzyme of the invention ( at pH 9.5; .quadrature. at pH 9.5 with 0.1% STPP added); and
FIG. 2 shows the relation between pH and proteolytic activity (% relative) of an enzyme of the invention.


DETAILED DISCLOSURE OF THE INVENTION

The present invention provides detergent compositions comprising proteases obtainable from a fungal strain of the genus Verticillium. Fungi belonging to the genus Verticillium are well known and described in the literature. Strains of Verticillium have been deposited and made available from various international depositary institutes, e.g. CBS No. 247.68; CBS No. 145.70; CBS No. 146.70; or CBS No. 464.88.
The proteases are obtainable by methods known and described in the literature, e.g. by cultivation of a protease producing strain of the genus Verticillium in a suitable nutrient medium, containing carbon and nitrogen sources and inorganic salts, followed by recovery of the desired enzyme, or may e.g. be produced by employing recombinant DNA technology.


The Proteases

In the context of this invention, suitable proteases are the proteases obtainable from strains of Verticillium, or routants or variants thereof or proteases having immunochemical properties identical or partially identical to a protease obtainable from a strain of Verticillium, e.g. V. bulbillosum.
By an enzyme variant or mutated enzyme is meant an enzyme obtainable by alteration of the DNA nucleotide sequence of the parent gene or its derivatives. The enzyme variant or mutated enzyme may be expressed and produced when the DNA nucleotide sequence encoding the enzyme is inserted into a suitable vector in a suitable host organism. The host organism is not necessarily identical to the organism from which the parent gene originated.
The immunochemical properties can be determined immunologically by cross-reaction identity tests. The identity tests can be performed by the well-known Ouchterlony double immunodiffusion procedure or by tandem crossed immunoelectrophoresis according to N. H. Axelsen; Handbook of Immunoprecipitation-in-Gel Techniques; Blackwe

REFERENCES:
patent: 5273896 (1993-12-01), Pedersen et al.

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