Detergent comprising isolated cellulase from bacillus ferm bp-34

Details Detergent comprising isolated cellulase from bacillus ferm bp-34 Detergent comprising isolated cellulase from bacillus ferm bp-34

1993-03-11

1994-05-24

Naff, David M.

Compositions

Water-softening or purifying or scale-inhibiting agents

252DIG12, 435209, 435832, 43525231, C11D 1700, C12N 942, C12N 120, C12N 100

Patent

active

053146379

ABSTRACT:
A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by a Bacillus strain FERM BP-3431 or a mutant strain thereof. A detergent comprising the cellulase, a builder and a surfactant is also disclosed.

REFERENCES:
patent: 3844890 (1974-10-01), Horikoshi et al.
patent: 3983002 (1976-09-01), Ohya et al.
patent: 4945053 (1990-07-01), Ito et al.
patent: 4962030 (1990-09-01), Kawai et al.
patent: 5045464 (1991-09-01), Ito et al.
patent: 5231022 (1993-07-01), Saito et al.
Tappi Journal 73 (12) 197-202, 1990, Pommier et al., "Using Enzymes to Improve the Process and the Product Quality in the Recycled Paper Industry", Part 2: Industrial Appliances.
Product Quality in the Recycled Paper Industry, Part 1: The Basic Laboratory Work, J. Bacteriology 124 (1) 48-54, 1975, Yoneda et al., "Mutation of Bacillus Subtilis Causing Hyperproduction of .alpha.-Amylase and Protease, and its Synergistic Effect".
Applied Environmental Microbiology 43 777-780, 1982, Teather et al., "Use of Congo Red-Polysaccharide Interactions in Enumeration and Characterization of Cellulolytic Bacteria from Bovine Rumen".
J. Gen. Microbiology 136 1327-1334, 1990, Ozaki et al., "Molecular Cloning and Nucleotide Sequence of a Gene for Alkaline Cellulase from Bacillus sp. KSM-635".
Appl. Microbiol. Biotechnol. 31 265-271, 1989, Huang et al., "Molecular Cloning and Expression of Multiple Cellulase Genes of Ruminococcus Flavefaciens Strain 186 in Escherichia coli".
J. Gen. Microbiology 131 3339-3345, 1985, Fukumori et al., "Purification and Properties of a Cellulase from Alkalophilic Bacillus sp. No. 1139".
Agric. Biol. Chem. 54 (1) 91-96, 1990, Shikata et al., "Alkaline Cellulases for Laundry Detergents: Production by Alkalophilic Strains of Bacillus and Some Properties of the Crude Enzymes".

Affiliated with

Also associated with

Rating

Detergent comprising isolated cellulase from bacillus ferm bp-34 does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detergent comprising isolated cellulase from bacillus ferm bp-34, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detergent comprising isolated cellulase from bacillus ferm bp-34 will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1971062