Compositions – Water-softening or purifying or scale-inhibiting agents
Patent
1990-05-04
1992-01-07
Lieberman, Paul
Compositions
Water-softening or purifying or scale-inhibiting agents
252551, 252552, 252553, 252555, 252556, 252558, 252DIG12, C11D 3386, C11D 742
Patent
active
050788981
DESCRIPTION:
BRIEF SUMMARY
This invention relates to a detergent composition comprising a protease and a lipase, and further to an enzymatic detergent additive comprising said enzymes.
BACKGROUND ART
Enzymatic detergent compositions are well known in the art. Enzymes of many types have been proposed for inclusion in detergent compositions, but the main attention has been focused on protease. Among the many proteases proposed for use in detergents, the following two are particularly relevant for this invention: amyloliquefaciens, see EP 130,756 (Genentech). and DK 86/5640 (Novo).
Lipases have also been proposed as detergent ingredients, but there is still relatively little prior art dealing with lipases for this use. Of particular relevance to this invention is the proposed use of Pseudomonas lipase, see e.g. GB 1,372,034 (Unilever) and EP 214,761 (Novo).
Detergents containing lipase and protease are also known. However, as the lipase is a protein it is liable to digestion and deactivation by the protease in the detergent solution. Thus, data in EP 205,208 (Unilever) and EP 206,390 (Unilever) demonstrate that the stability of lipase from Pseudomonas fluorescens in detergent solution is seriously reduced by addition of protease from Bacillus licheniformis (Alcalase.RTM.) or from alkalophilic Bacillus sp. (Savinase.RTM. and Esperase.RTM., trade marks of Novo Industri A/S).
Further, EP 130,064 (Novo), EP 214,761 (Novo) and WO 87/00859 (Gist-Brocades N.V.) disclose detergents with protease of Bacillus licheniformis (described as ALCALASE.RTM. and MAXATASE.RTM., trade names of Novo and Gist-Brocades, respectively) and lipase of Fusarium oxysporum, Pseudomonas cepacia, Ps. pseudoalcaligenes or Ps. stutzeri, Stability data have not been published, but data in examples of this specification show that the stability of the lipase in these combinations is poor due to the influence of the protease.
It is the object of the invention to provide detergent compositions containing both lipase and protease, such that: fatty and proteinaceous soiling, respectively detergent, and detergent, and that hence the detergency towards fatty soiling is not significantly reduced by the protease.
Surprisingly, we have now discovered that all these objectives can be achieved by selecting a certain group of lipases and a certain group of proteases. Specifically, this combination of lipase and protease shows better lipase stability in detergent solution than the prior art.
STATEMENT OF THE INVENTION
The invention provides a detergent composition comprising a protease and a lipase. The protease is either Subtilisin Novo, a variant thereof (of a kind to be defined below) or a Fusarium protease. The lipase is derived from Pseudomonas.
The invention also provides an enzymatic detergent additive comprising said protease and said lipase.
DETAILED EXPLANATION OF THE INVENTION
Protease
The class of proteases that can be used in the present invention comprises proteases of Fusarium sp., Subtilisin Novo and certain variants of the latter.
Protease for use in the invention may be produced by cultivation of a strain of Fusarium sp., especially F. oxysporum and F. solani. Preferred strains include DSM 2672, IFO 5880, ATCC 659 and other strains listed in U.S. Pat. No. 3,652,399 (Takeda), as well as mutants and variants of these. Cultivation of the strains and recovery of protease may be done according to principles known in the art, U.S. Pat. No. 3,652,399.
Preferred Fusarium proteases for use in the invention are active in the pH range 7-12 especially 8-10.5, and most preferably they have pH optimum in such range.
The strain DSM 2672 was deposited on 6 June 1983 under the terms of the Budapest Treaty. It has been identified as F. oxysporum. The other strains are freely available to the public. DSM indicates Deutsche Sammlung von Mikroorganismen, West Germany (DSM), IFO stands for Institute of Fermentation, Osaka (IFO), and ATCC indicates American Type Culture Collection, U.S.A.
Subtilisin Novo is an alkaline protease from Bacillus amyloquefaciens. It has also been de
REFERENCES:
patent: 3652399 (1972-03-01), Isono et al.
patent: 3655570 (1972-04-01), Isono et al.
patent: 4707291 (1987-11-01), Thom et al.
patent: 4760025 (1988-07-01), Estell et al.
patent: 4876024 (1989-10-01), Enomoto et al.
patent: 4927558 (1990-05-01), Aaslyng et al.
patent: 4933287 (1990-06-01), Farin et al.
patent: 4980288 (1990-12-01), Bryan et al.
Beadles-Hay A.
Lambiris Elias J.
Lieberman Paul
Novo Nordisk A S
Zelson Steve T.
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