Compositions – Fluent dielectric – N-containing
Patent
1993-04-06
1995-02-14
Skane, Christine
Compositions
Fluent dielectric
N-containing
25217412, 252DIG12, 435219, 435220, 435221, 435222, C11D 112, C11D 3386
Patent
active
053893077
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention relates to detergent compositions. More specifically, the invention relates to detergent compositions containing alkyl sulphate and one or more subtilisins or subtilisin variants, which subtilisins or subtilisin variants either in position 195 hold a glutamic acid residue, or in position 222 hold an alanine residue, or both.
BACKGROUND ART
Alkyl sulphate (AS) is an anionic surfactant used in various detergent compositions, often in mixtures with other anionic surfactants, especially linear alkylbenzene sulfonates (LAS). Anionic surfactants are known to provide excellent cleaning performance, and in numerous patent publications the use of AS as detergent ingredient is described, vide e.g. EP 219,314; EP 220,024; and EP 328,184.
In detergent compositions the use of AS is solely due to its superior detergency performance. It was certainly not to be anticipated that AS should have any positive influence on some detergent enzymes, while not on others. However, it has now surprisingly been found that AS exerts a positive influence on the performance of a specific group of detergent enzymes.
SUMMARY OF THE INVENTION
It has now been found that AS, when present, is able to increase the washability of certain detergent enzymes. These detergent enzymes belong to a group of subtilisins holding a glutamic acid residue and/or an alanine residue at specific positions.
Accordingly, the present invention provides a detergent composition comprising alkyl sulphate and one or more subtilisins or subtilisin variants, which subtilisins or subtilisin variants in position 195 hold a glutamic acid residue, and/or in position 222 hold an alanine residue.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated by reference to the accompanying drawings, in which FIGS. 1-3 show a comparison of the wash performance of two detergent enzymes (A: Subtilisin 309/G 195E/M222A; and B: Subtilisin 309) in three different model detergents (Model I-III, respectively, cf. the Example; Grass juice soiled cotton, 10 min. at 20.degree. C., 3.degree.dH, and pH 10).
DETAILED DISCLOSURE OF THE INVENTION
Experiments involving various detergent enzymes in various detergent compositions have now demonstrated that certain subtilisins show increased wash performance in the presence of alkyl sulphate. More investigations on this subject indicated that a glutamic acid residue and/or an alanine residue at certain specific positions are responsible for this effect.
In respect to definitions of subtilisins, reference is made to e.g. International Patent Publication Nos. WO 89/06279 and WO 91/00345. In these publications a survey of the amino acid sequence of various subtilisins is given. By alignment of the amino acid sequences of various subtilisins along with Subtilisin BPN' (Subtilisin NOVO) it is possible to allot a number to the amino acid residue position in any subtilisin, to the number of the analogous amino acid position in Subtilisin BPN', and hence to indicate the position of an amino acid residue in a subtilisin enzyme unambiguously.
Thus, referring to this numbering system, in the context of this invention referred to as "BPN' numbering", the above mentioned investigations indicate that a glutamic acid residue in position 195 and/or an alanine residue in position 222 (adjacent to the active serine at position 221 ) are responsible for the increased wash performance, when AS is present.
By a subtilisin variant, a mutated subtilisin, or a subtilisin derivative, is meant a subtilisin obtainable by alteration of a DNA nucleotide sequence of the parent gene or its derivatives. The subtilisin variant or mutated subtilisin may be expressed and produced when the DNA nucleotide sequence encoding the subtilisin is inserted into a suitable vector in a suitable host organism.
The subtilisin variant, mutated subtilisin or subtilisin derivative may also be a subtilisin that by way of e.g. protein engineering or by chemical or biological treatment is altered to produce a functionally equivalent derivat
REFERENCES:
patent: 3790482 (1974-02-01), Jones
patent: 4760025 (1988-07-01), Estell et al.
patent: 4914031 (1990-04-01), Zukowski
patent: 5185258 (1993-02-01), Caldwell et al.
Aaslyng Dorrit A.
Lindegaard Poul
Fries Kery
Lambiris Elias J.
Novo Nordisk A S
Skane Christine
Zelson Steve T.
LandOfFree
Detergent composition comprising an alkyl sulfate and a subtilis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detergent composition comprising an alkyl sulfate and a subtilis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detergent composition comprising an alkyl sulfate and a subtilis will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-285626