Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-05-30
2009-11-10
Chunduru, Suryaprabha (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S006120, C435S091200
Reexamination Certificate
active
07615620
ABSTRACT:
The present invention provides a detection system for a PCR process using FRET which comprises at least two single-labelled Oligonucleotide sequences of differing Tm that hybridize to one another in free solution to form a fluorescent quenched pair, that upon introduction of a complementary sequence to one or both sequences generates a measurable signal, one of the sequences being of a Tm that is below the Ta of the PCR process, the other not being below the Ta of the PCR process.
REFERENCES:
patent: 5866336 (1999-02-01), Nazarenko et al.
patent: 6326145 (2001-12-01), Whitcombe et al.
patent: 6699975 (2004-03-01), Reed et al.
patent: 199 34 084 (2001-01-01), None
patent: 102 50 948 (2004-05-01), None
patent: 0 909 823 (1999-04-01), None
patent: 1403382 (2004-03-01), None
patent: 013399 (1995-05-01), None
patent: 049293 (1999-09-01), None
patent: 041549 (2000-07-01), None
patent: 030946 (2002-04-01), None
patent: 062422 (2003-07-01), None
patent: 003136 (2004-01-01), None
Myakishev, MV et al. High-throughput SNP genotyping by allele-specific PCR with universal energy-transfer-labelled primers. Genome Research, vol. 11, pp. 163-169, 2001.
Kong, D-M., et al., “Simulation of TaqMan by two single-labelled probes,” Chem. Commun. , 2584-2588, 2002.
Kong, D-M., et al., “Use of duplex probes simulating TaqMan to detect hepatitis B virus,” New J. Chem., 2003, vol. 27, p. 721-726.
Koo, K., et al., “Detection of Listeria monocytogenes from a Model Food by Fluorescence Resonance Energy Transfer-Based PCR with an Asymmetric Fluorogeic Probe Set,” Appl. Env. Micriobio., 2003, p. 1082-1088.
Koo, K., and Jaykus, L.-A., “Detection of single nucleotide polymorphisms within the Listeria genus using an asymmetric fluorogenic probe set and fluorescence resonance energy transfer based-PCR,” Lett. Appl. Micriobiol., 2002, vol. 35, 513-517.
Solinas, A., et al., “Duplex Scorpior primers ins SNP anaysis and FRET applications,” Nucleic Acid Rsch., 2001, vol. 29, No. 20 e96 (9 pages).
Purcell, P., Search Report in respect of GB0510979.8, searched Mar. 31, 2006, The Patent Office (UK).
Purcell, P., Search Report in respect of GB0610653.8, searched Sep. 1, 2006, The Patent Office (UK).
Santagati, F., Search Report in respect of EP 06252796, searched Jul. 19, 2006, European Patent Office.
F. Santagati, European Patent Office, Communication pursuant to Article 94(3) EPC, in respect of Application No. 06 252 796.5—1222, mailed Apr. 17, 2008.
Chunduru Suryaprabha
KBiosciences Limited
Ruben Bradley N.
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