Detection reagent for thermostable direct hemolysin-related...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S006120, C435S091200, C536S023100, C536S024300, C536S024320

Reexamination Certificate

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07495094

ABSTRACT:
A detection reagent for detecting thermostable direct hemolysin-related hemolysin (TRH) gene in an amplification process which specifically amplifies TRH1 and TRH2 RNA, which reagent comprises a first primer having a sequence complementary to a specific sequence of an RNA derived from the TRH gene, and a second primer having a sequence homologous to said specific sequence.

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CD-ROM containing an unbridged electronic version of the article on the “Preparaion and Utilization of Isolated and Purified Oligonucleotides” by Dr. Andrew Chin.
Masamichi Kishishita, et al., “Sequence Variation in the Thermostable Direct Hemolysin-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus”, Applied and Environmental Microbiology, vol. 58, No. 8, XP-009012050, Aug. 1992, pp. 2449-2457.
Jun Tada, et al., “Detection of the Thermostable Direct Hemolysin Gene (tdh) and the Themostable Direct Hemolysin-related Hemolysin Gene (trh) of Vibrio Parahaemolyticus by Polymerase Chain Reaction”, Molecular and Cellular Probes, vol. 6, Dec. 1992, pp. 477-487.

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