Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-01-02
2007-01-02
Smith, Lynette R. F. (Department: 1645)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S004000, C435S007920, C435S007930, C435S007940, C435S007950, C435S174000, C435S283100, C435S287100, C435S287200, C435S287700, C435S288300, C435S402000, C436S501000, C436S506000, C436S506000, C436S507000, C436S543000, C436S546000
Reexamination Certificate
active
09063978
ABSTRACT:
A microscale binding assay, analyte binding array, and kits are disclosed, which exploit the mass action law to harvest analyte from a liquid sample. This is achieved by fabrication of sorbent zones having up to ten times the binding capacity per unit area generally obtained on polystyrene microtiter plates. The resulting arrays substantially deplete the liquid solution of analyte during incubation. Accordingly, the assays respond to total mass of analyte in the sample, not analyte concentration. This approach, coupled with direct fluorescence detection in the NIR, yields maximal signal intensity and low background for optimal sensitivity.
REFERENCES:
patent: 4385126 (1983-05-01), Chen et al.
patent: 4745072 (1988-05-01), Ekins et al.
patent: 4954435 (1990-09-01), Krauth
patent: 5171695 (1992-12-01), Ekins
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5432099 (1995-07-01), Ekins
patent: 5453505 (1995-09-01), Lee et al.
patent: 5512659 (1996-04-01), Ullman et al.
patent: 5516635 (1996-05-01), Ekins et al.
patent: 5639423 (1997-06-01), Northrup et al.
patent: 134215 (1983-08-01), None
patent: 271974 (1987-08-01), None
patent: 304202 (1988-08-01), None
patent: 0 134 215 (1989-10-01), None
patent: 0 304 202 (1992-07-01), None
patent: 0 271 974 (1993-03-01), None
patent: WO 84/01031 (1984-03-01), None
patent: WO 88/01058 (1988-02-01), None
patent: WO 89/01157 (1989-02-01), None
patent: WO 93/08472 (1993-04-01), None
patent: WO 95/18376 (1995-07-01), None
patent: WO 95/18377 (1995-07-01), None
patent: WO 95/24649 (1995-09-01), None
patent: WO 95/35505 (1995-12-01), None
patent: WO 9732212 (1997-09-01), None
Ekins et al. 1989. J. of Biolum and Chemilum. 4:59-79.
Ekins et al. 1990. Ann. Biol. Clin. 48:655-666.
Ekins et al. 1990. J. of Clin. Immuno. 13(4):169-181.
Ekins et al. 1989. Analytica Chimica Acta. 73-96.
Ekins, Rober et al; “Development of Microspot Multi-Analyte Ratiometric Immunoassay Using Dual Fluorescent-Labelled Antibodies” Analytica Chimica Acta 227 (1989).
Ekins, R. et al; “Multianalyte Microspot Immunoassay, The Micro-analytical ‘compact disk’ of the Future”, Ann Biol Clin, V. 50, pp. 337-353 (1992).
Kakabakos, S. et al; Multianalyte Immunoassay Based on Spatially Distinct Fluorescent Areas Quantified by Laser-Excited Solid-Phase Time Resolved Fluorometry; Clin. Chem. 38/3, pp. 338-342 (1992).
Sigal, Nolan et al; “Approaches and Technologies for Screening Large Combinatorial Libraries”; Combinatorial Chemistry and Molecular Diversity in Drug Discovery, pp. 433-443 (1998).
Ekins, R.P., et al., “Developing multianalyte assays”, Reviews—Trends in Biotechnology, p. 89 (1992).
Ekins, R., et al., “High specific Activity Chemiluminescent and Fluorescent Markers: their Potential Application to High Sensitivity and ‘Multi-analyte’ Immunoassays”,J. of Bioluminescence and Chemiluminescence, 4:59 (1989).
Ekins, R., et al., “Development of Microspot Multi-Analyte Ratiometric Immunoassay Using Dual Fluorescent-Labelled Antibodies”,Analytica Chimica Acta, 227:73 (1989).
Ekins, R.P., “Multi-analyte immunoassay”,Journal of Pharmaceutical&Miomedical Analysis, 7(2):155 (1989).
Ekins, R., et al., “Fluorescence spectroscopy and its application to a new generation of high sensitivity, multi-microspot, multianalyte, immunoassay”,Clinica Chimica Acta, 194:91 (1990).
Ekins, R., et al., “Multispot, multianalyte, immunoassay”,Ann. Biol. Clin., 48:655 (1990).
Ekins, R., “Time resolved fluorescenct spectroscopy and its application to a new generation of high sensitivity, muti analyte, ‘microspot’ immunoassays”Fresenius J. Anal. Chem., 337:20 (1990).
Ekins, R.P., et al., “Multianalyte Immunoassay: The Immunological ‘Compact Disk’ of the Future”,J. Clin. Immunoassay, 13(4):169 (1990).
Ekins, R.P., “Competitive, Noncompetitive, and Multi-Analyte Microspot Immunoassays”,Immunochemistry of Solid-Phase Immunoassay, Chapt. 6, p. 105 (1991).
Ekins, R.P., et al., “Multianalyte Microspot Immunoassay-Microanalytical ‘Compact Disk’ of the Future”,Clin. Chem., 37(11):1955 (1991).
Ekins, R., “Principles of Non-Competitive Methods”,Principles of Immunoassays, Chapt. 3.3.2, Pub.: VCH, New York, p. 227 (1993).
Ekins, R., et al., “Multianalyte Testing[Letter]”,Clin. Chem., 39(2):369 (1993).
Ekins, R.P., “New perspectives in radioimmunoassay”,Nuclear Medicine Communications, 14:721 (1993).
Ekins, R., et al., “Multianalyte Microspot Immunoassay”,Anal. Proc., 30:488 (1993).
Ekins, R., “Immunoassay: recent developments and future directions”,Nucl. Med. Biol., 21(3):495 (1994).
Kakabakos, S.E., et al., “Multianalyte Immunoassay Based on Spatially Distinct Fluorescent Areas Quantified by Laser-Excited Solid-Phase Time-Resolved Fluorometry”,Clin. Chem., 38 (3):338 (1992).
Patel, N., “Application of Ambient Analyte Immunoassays for Pesticide Multi-Residue Analysis”, Proceedings ofFood Safety and Quality Assurance: Applications of Immunoassay Systems, Ed. M.R.A. Morgan; Elsevier Applied Science, London/New York, p. 129 (1991).
Brett a. Stillman et al.;Expression Microarray Hybridization Kinetics Depend on Length of the Immobilized DNA but Are Independent of Immobilization Substrate; Analytical Biochemistry, 295; 149-157 (2001).
Kim E. Sapsford et al.;Kinetics of Antigen Binding to Arrays of Antibodies in Different Sized Spots; Anal. Chem. 2001, 73, 5518-5524.
Cercek Bibijana
Dodson Charles L.
Liu Yagang
Obremski Robert J.
Shaomin Zhou
Beckman Coulter Inc.
Hines J.
Hogan & Hartson LLP
Smith Lynette R. F.
LandOfFree
Detection of very low quantities of analyte bound to a solid... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Detection of very low quantities of analyte bound to a solid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of very low quantities of analyte bound to a solid... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3732673