Detection of vancomycin-resistant enterococcus spp.

Details Detection of vancomycin-resistant enterococcus spp. Detection of vancomycin-resistant enterococcus spp.

2006-07-11

2006-07-11

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091100, C435S006120

Reexamination Certificate

active

07074598

ABSTRACT:
The invention provides methods to detect vancomycin-resistantenterococciin biological samples using real-time PCR. Primers and probes for the detection of vancomycin-resistantenterococciare provided by the invention. Articles of manufacture containing such primers and probes for detecting vancomycin-resistantenterococciare further provided by the invention.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4996143 (1991-02-01), Heller et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5565322 (1996-10-01), Heller
patent: 5683896 (1997-11-01), Hartley et al.
patent: 5702895 (1997-12-01), Matsunaga et al.
patent: 5837452 (1998-11-01), Clark et al.
patent: 5849489 (1998-12-01), Heller
patent: 5945313 (1999-08-01), Hartley et al.
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6162603 (2000-12-01), Heller
patent: 6593093 (2003-07-01), Uhl et al.
patent: 269 764 (1988-06-01), None
patent: 0 338 591 (1989-10-01), None
patent: 0 526 876 (1993-02-01), None
patent: 1 045 033 (2000-10-01), None
patent: 1 160 333 (2001-12-01), None
patent: WO 97/46707 (1997-12-01), None
patent: WO 97/46712 (1997-12-01), None
patent: WO 97/46714 (1997-12-01), None
patent: WO 98/48046 (1998-10-01), None
patent: WO 99/19466 (1999-04-01), None
patent: WO 99/45155 (1999-09-01), None
patent: WO 00/37646 (2000-06-01), None
patent: WO 00/70096 (2000-11-01), None
patent: WO 01/12803 (2001-02-01), None
patent: WO 01/23604 (2001-04-01), None
patent: WO 02/18660 (2002-03-01), None
patent: WO 02/34771 (2002-05-01), None
patent: WO 02/61390 (2002-08-01), None
patent: WO 02/92818 (2002-11-01), None
patent: WO 03/25216 (2003-03-01), None
patent: WO 03/068918 (2003-08-01), None
patent: WO 03/93306 (2003-11-01), None
Pitt et al . Molecular bacteriology: a diagnostic tool for the millennium. Journal of clinical pathology, vol. 53, pp. 71-75, 2000.
GenBank Accession No. AF310954.
GenBank Accession No. AY035705.
GenBank Accession No. M97297.
GenBank Accession No. U72704.
GenBank Accession No. U94528.
GenBank Accession No. X56895.
GenBank Accession No. 270527.
Patel et al., “Multiplex PCR Detection ofvanA, vanB, vanC-1, andvanC-2/3Genes in Enterococci,”Journal of Clinical Microbiology, 1997, 35:703-707.
D'Agata et al., “High Rate of False-Negative Results of the Rectal Swab Culture Method in Detection of Gastrointestinal Colonization with Vancomycin-Resistant Enterococci,”Clinical Infectious Diseases, 2002, 34:167-72.
Sloan et al., “Evaluation of a Combined LightCycler Assay for the Detection of vanA, vanB, and vanB-2/3 Genes in Enterococci,”Abstracts of the 102ndGeneral Meeting of the American Society for Microbiology, Salt Lake City, Utah, May 19-23, 2002, C-242.
Bassler et al., “Use of a Fluorogenic Probe in a PCR-Based Assay for the Detection ofListeria monocytogenes,” Applied and Environmental Microbiology, 1995, 61(10):3724-3728.
Espy et al., “Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR,”J. Clin. Microbiol., 2000, 38(2):795-799.
Brink et al., “Nucleic Acid Sequence-Based Amplification, A New Method for Analysis of Spliced and Unspliced Epstein-Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR,”J. Clin. Microbiol., 1998, 36(11):3164-3169.
Caplin et al., “LightCycler™ hybridization probes; The most direct way to monitor PCR amplification for quantification and mutation detection,”Biochemica, 1999, 1:5-8.
Espy et al., “Quantification of Epstein-Barr Virus (EBV) viral Load in Transplant Patients by LightCycler PCR,”Abstracts of the General Meeting of the American Society for Microbiology, 101stGeneral Meeting, May 20-24, 2001, 101:182, Abstract No. C-148.
Espy et al., “Diagnosis of Varicella-Zoster Virus Infections in the Clinical Laboratory by LightCycler PCR,”J. Clin. Microbiol., 2000, 38(9):3187-3189.
Espy et al., “Diagnosis of Herpes Simplex Virus Infections in the Clinical Laboratory by LightCycler PCR,”J. Clin. Microbiol., 2000, 38(2)795-799.
Espy et al., “Detection of Smallpox Virus DNA by LightCycler PCR,”J. Clin. Microbiol., 2002, 40(6):1985-1988.
Sample et al., “Two Related Epstein-Barr Virus Membrane Proteins are Encoded by Separate Genes,”J. Virol., 1989, 63(2):933-937.
Smith, “Application of Lightcycler Real Time PCR in Clinical Virology,”Clin. Chem. Lab. Med., 2001, Special Supplement, 39:S60, Abstract No. ISW14-2.
Telenti et al., “Detection of Epstein-Barr Virus by Polymerase Chain Reaction,”J. Clin. Microbiol., 1990, 28(10):2187-2190.
Al-Robaiy et al., “Rapid Competitive PCR Using Melting Curve Analysis for DNA Quantification,”BioTechniques, 2001, 31:1382-1388.
Bélanger et al., “Rapid Detection of Shiga Toxin-Producing Bacteria in Feces by Multriplex PCR with Molecular Beacons on the Smart Cycler,”J. Clin. Microbiol., 2002, 40:1436-1440.
Bellin et al., “Rapid Detection of EnterohemorrhagicEscherichia coliby Real-Time PCR with Fluorescent Hybridization Probes,”J. Clin. Microbiol., 2001, 39:370-374.
Chen et al., An Automated Fluorescent PCR Method for Detection of Shiga Toxin-ProducingEscherichia coliin Foods,:Appl. Environ. Microbiol., 1998, 64:4210-4216.
Didenko, “DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications,”BioTechniques, 2001, 31:1106-1121.
Ramotar et al., “Direct Detection of Verotoxin-ProducingEscherichia coliin Stool Samples by PCR,”J. Clin. Microbiol., 1995, 33:519-524.
Livak et al., “Oligonucleotides with Fluorescent Dyes at Opposite Ends Provide A Quenched Probe System Useful for Detecting PCR Product and Nucleic Acid Hybridization,”Genome Research, 1995, 4:357-362.
Arthur et al., “Enterococcus faeciumtransposon Tn1546 transposase, resolvase, vanR, vanS, vanH, vanA, vanX, vanY and teicoplanin resistance protein (vanZ) genes, complete cds,” 1993, database accession No. M97297.
Grisold et al., “Detection of Methicillin-ResistantStaphylococcus aureusand Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR,”J. Clin. Microbiol., 2002, 40:2392-2397.
Huletsky et al., “Rapid Detection of Vancomycin-Resistant Enterococci Directly from Rectal Swabs by Real-Time PCR Using the Smart Cycler,”Abstracts of the Interscience Conference on Antimicrobiol Agents and Chemotherapy, Chicago, Illinois, Sep. 22-25, 2001, 41:409 (Abstract K-1195).
Ito et al., “Staphylococcus aureusDNA, type-I staphylococcal cassette chromosome mec,” 1999, database accession No. AB033763.
“LightCycler-FastStart DNA Master Hybridization Probes,” 1999 Roche Diagnostics GmbH Technical Manual, retrieved from the internet on Feb. 6, 2004: http://www.roche-applied-science.com.
Palladino et al., “Real-time PCR for the rapid detection ofvanAandvanBgenes,”Diagnostic Microbiology and Infectious Disease, 2003, 45:81-84.
Palladino et al., “Rapid Detection ofvanAandvanBGenes Directly from Clinical Specimens and Enrichment Broths by Real-Time Multiplex PCR Assay,”J. Clin. Microbiol., 2003, 41:2483-2486.
Patel et al., “Enterococcus faecalisvancomycin resistance protein vanB gene, partial cds,” 1997, database accession No. U72704.
Patel et al., “Enterococcus faeciumvancomycin resistance protein B (vanB) gene, partial cds,” 1997, database accession No. U94528.
Petrich et al., “Direct detection ofvanAandvanBgenes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR,”Molecular and Cellular Probes, 1999, 13:275-281.
Reischl et al., “Rapid Identification of Methicillin-ResistantStaphylococcus aureusand Simultaneous Species Confirmation Using Real-Time Fluorescence PCR,”J. Clin.

Affiliated with

Also associated with

Rating

Detection of vancomycin-resistant enterococcus spp. does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Detection of vancomycin-resistant enterococcus spp., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of vancomycin-resistant enterococcus spp. will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3592767