Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target
Reexamination Certificate
2006-10-03
2006-10-03
Ceperley, Mary E. (Department: 1641)
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Cell membrane or cell surface is target
C435S029000, C435S173600, C436S164000, C436S172000, C436S800000, C436S805000
Reexamination Certificate
active
07115401
ABSTRACT:
Methods and compositions are provided for determining the potential of a membrane. In one aspect, the method comprises:(a) introducing a first reagent comprising a hydrophobic fluorescent ion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane, as described by the Nernst equation,(b) introducing a second reagent which labels the first face or the second face of the membrane, which second reagent comprises a chromophore capable of undergoing energy transfer by either (i) donating excited state energy to the fluorescent ion, or (ii) accepting excited state energy from the fluorescent ion,(c) exposing the membrane to radiation;(d) measuring energy transfer between the fluorescent ion and the second reagent, and(e) relating the energy transfer to the membrane potential.Energy transfer is typically measured by fluorescence resonance energy transfer. In some embodiments the first and second reagents are bound together by a suitable linker.In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.
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Gonzalez, III Jesus E.
Tsien Roger Y.
Ceperley Mary E.
The Regents of the University of California
Townsend and Townsend / and Crew LLP
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